Single cell RNA-seq by mostly-natural sequencing by synthesis
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ABSTRACT: Massively parallel single cell RNA-seq (scRNA-seq) for diverse applications, from cell atlases to functional screens, is increasingly limited by sequencing costs, and large-scale low-cost sequencing can open many additional applications, including patient diagnostics and drug screens. Here, we adapted and systematically benchmarked a newly developed, mostly-natural sequencing by synthesis method for scRNA-seq. We demonstrate successful application in four scRNA-seq case studies of different technical and biological types, including 5’ and 3’ scRNA-seq, human peripheral blood mononuclear cells from a single individual and in multiplex, as well as Perturb-Seq. Our data show comparable results to existing technology, including compatibility with state of the art scRNA-seq libraries independent of the sequencing technology used – thus providing an enhanced cost-effective path for large scale scRNA-seq.
ORGANISM(S): Homo sapiens
PROVIDER: GSE197452 | GEO | 2022/05/31
REPOSITORIES: GEO
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