Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA Sequencing Analysis of the gonadal adipose tissue and liver from PEP Wild Type, heterozygotes and knock out mice.

ABSTRACT: Purpose: Peptidase D (PEP) plays a vital role in collagen turnover by degrading proline-containing dipeptides but its specific functional relevance in adipose tissue is unknown. The goal of this study is to compare transcriptome profiling (RNA-seq) in the gonadal adipose tissue and liver of wild type mice and mice where PEP has been ablated either partially (heterozygotes) or totaly (knock out).

ORGANISM(S): Mus musculus

PROVIDER: GSE198358 | GEO | 2022/03/13

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

RNA Sequencing Analysis of the gonadal adipose tissue and liver from PEP Wild Type, heterozygotes and knock out mice.

Project description:RNA Sequencing Analysis of the gonadal adipose tissue and liver from PEP Wild Type, heterozygotes and knock out mice.

| PRJNA814684 | ENA

Caveolin-1 knockout liver and gonadal adipose tissue

Project description:mRNA expression was compared in between wild type and caveolin-1 knockout livers mRNA expression was compared in between wild type and caveolin-1 knockout gonadal adipose tissue RNA was isolated from livers from male mice in at the light phase RNA was isolated from gonadal adipose tissues from male mice in at the light phase

2012-01-31 | E-GEOD-35431 | biostudies-arrayexpress

Caveolin-1 knockout liver and gonadal adipose tissue

2012-01-31 | GSE35431 | GEO

Gonadal Adipose Tissue Profiling In HMDP (high-fat/high-sucrose diet)

Project description:Identify genes in the gonadal adipose tissue whose expression is under genetic regulation in the Hybrid Mouse Diversity Panel (HMDP). The HMDP comprises classical inbred and recombinant inbred wild type mice. The RMA values of genes were used for genome wide association as described in Parks et al Cell Metabolism 2015. These data are used to identify candidate genes at loci associated with obesity and dietary responsiveness. GWAS for expression of gonadal adipose tissue in inbred strains fed chow diet for 8 weeks followed by high-fat/high-sucrose diet 8 weeks

2015-01-08 | E-GEOD-64768 | biostudies-arrayexpress

Subcutaneous and gonadal adipose tissue transcriptome differences in lean and obese female dogs

Project description:Adipose, once considered an inert storage depot, is now known to be an active endocrine tissue involved in total body homeostasis and metabolism, which exerts effects on multiple systems including food intake, immune function, and blood glucose regulation. Adipose tissue depots are known to have unique metabolic and gene expression profiles in vivo and when cultured in vitro. Differences in adipose tissue depot function could be important in determining chronic disease risk. Few comparisons of depot gene expression have been performed in the dog. Utilizing microarray technology, our objective was to identify differentially expressed genes and enriched functional pathways between subcutaneous and gonadal adipose of lean and obese dogs. Subcutaneous and gonadal adipose tissue samples were collected from 9 intact female beagles (4 yr-old; 4 lean controls; 5 obese ad libitum-fed) after 24 wk of ad libitum feeding.

2012-01-15 | E-GEOD-29280 | biostudies-arrayexpress

Analysis of gonadal fat from male control and adipocyte-specific LAMA4 knockout mice

Project description:To determine the transcriptomic effects of LAMA4 loss on adipose tissue structure and gene expression in two dietary paradigms, we assessed gonadal white adipose tissue of male control and adipocyte-specific LAMA4 knockout mice using a Quant-Seq strategy.

2022-08-21 | GSE179138 | GEO

Next Generation Sequencing and Analysis of the Transcriptomes of RAW264.7 cells pretreated with PEP and PBS.

Project description:We demonstrate that phosphoenolpyruvate (PEP) generally inhibits viral infection in vitro and in vivo. In order to further explore whether PEP controls viral replication through a general mechanism such as enhancing innate response, and if not, what's the critical downstream target(s) of PEP, we performed RNA sequencing (RNA-seq) analysis on the RAW264.7 cells that were pretreated with 250 μM PEP or PBS for 2 hours, and then infected with or without vesicular stomatitis virus (VSV) for 8 hours.

2023-03-16 | GSE223228 | GEO

Dose-dependent changes in gonadal white adipose tissue in TCDD-elicited steatohepatitis

Project description:Bulk RNA-sequencing of 27 C57BL/6 gonadal white adipose tissue samples where ovariectomized females were gavaged 4 days for 92 days with 0.01 to 30 µg/kg TCDD or sesame oil vehicle (n = 3) per dose.

2024-10-29 | GSE272683 | GEO

Gene expression profiles of p16high and p16low cells in the gonadal adipose tissue of 18 month old mice [scRNA_Adipose_CD45]

Project description:We used scRNA-seq analysis to investigate the cell-to-cell communication in the gonadal adipose tissue of aging mice.

2024-05-31 | GSE264000 | GEO

Aspergillus niger Prolyl endoprotease (An-PEP) for Hydrogen-Deuterium Exchange Mass Spectrometry and Protein Structural Studies

Project description:To monitor the structural integrity of therapeutic proteins, hydrogen-deuterium exchange mass spectrometry (HDX-MS) is increasingly utilized in the pharmaceutical industry. The successful outcome of HDX-MS analyses depends on the sample preparation conditions, which involve the rapid digestion of proteins at 0Â°C and pH 2.5. Very few proteases are known to withstand such harsh conditions, with pepsin being the best-known exception, even though its activity is also strongly reduced at 0Â°C. Here, we evaluate the usage of a Prolyl-endopeptidase from Aspergillus niger (An-PEP) for HDX-MS. What makes this protease very attractive is its ability to cleave the hardest to digest amino acid, proline. To our surprise, and in contrast to previous reports, An-PEP activity was found optimal around pH 2.5 and could be further enhanced by urea up to 40%. Under typical HDX-MS conditions and using small amounts of enzyme, An-PEP generated an equivalent number of peptides as pepsin, as exemplified by using the two model systems; tetrameric human hemoglobin (Hb) and human IgG4. Interestingly, because An-PEP peptides are shorter than pepsin-generated peptides, higher sequence resolution could be achieved, especially for Pro-containing protein regions in the alpha subunit of Hb, revealing new protected Hb regions that were not observed with pepsin. Due to its Pro-preference and resistance to low pH, we conclude that An-PEP is an archetype enzyme for HDX-MS, highly complementary to pepsin, and especially promising for structural studies on Pro-rich proteins (PRPs) or proteins containing Pro-rich binding domains involved in cellular signaling.

2017-07-12 | PXD006766 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data