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Longitudinal transcriptomic analysis of mouse sciatic nerve reveals pathways associated with age-related muscle pathology

ABSTRACT: Our objective was to identify early changes in gene expression related to the development of sarcopenia via transcriptomic profiling of aging sciatic nerves in vivo. Sciatic nerve mRNA profiles of C57BL/6JN mice aged 5 (n = 6), 18 (n = 5), 21 (n = 6), and 24 months (n = 5) old were generated by RNA sequencing using an Illumina HiSeq4000. The sequence reads that passed quality filters were mapped to the mouse reference genome using STAR and featureCounts. Differential expression analysis was performed using DESeq2. Differentially expressed genes were validated using quantitative reverse transcription PCR (qRT-PCR) using SYBR Green assays. We detected 51 significant differentially expressed genes in sciatic nerve of 18-month-old mice compared to 5-month-old mice. These genes were associated with the AMPK signaling pathway, biosynthesis and metabolic pathways, and circadian rhythm. These early molecular changes shed a new light on biological processes in peripheral nerve that may be implicated in sarcopenia initiation and pathogenesis. Future studies are warranted to confirm the disease modifying and/or biomarker potential of the key changes we report here.

ORGANISM(S): Mus musculus

PROVIDER: GSE198669 | GEO | 2023/03/13

REPOSITORIES: GEO

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