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Derivation of cochlear organoids from human pluripotent stem cells reveals tight transcriptome-structure coupling during organogenesis

ABSTRACT: A balance of morphogen gradients during embryogenesis is thought to determine the identity of inner ear end organs. We applied this developmental principle to aggregates of human pluripotent stem cells and found that modulations of Sonic Hedgehog and WNT signaling promote stem cell-derived otic progenitors to express ventral otic markers. Strikingly, these ventralized otic progenitors gave rise to hair cells with short hair bundles comprised of stereocilia arrayed in a geometry reminiscent of cochlear hair cells. Moreover, these ventralized hair cells expressed multiple markers defining outer or inner hair cells in the cochlea. These results reveal that early morphogenic signals are sufficient for not only establishing cochlear gene expression, but also defining structural properties pertaining to the cochlear sensory epithelium.

ORGANISM(S): Homo sapiens

PROVIDER: GSE200629 | GEO | 2022/04/14

REPOSITORIES: GEO

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Mapping the developmental potential of mouse inner ear organoids at single-cell resolution

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Gene expression of differentiating hESCs into otic progenitors

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RNA-seq analysis of Barhl1 locus mutant mouse embryonic stem cell (mESC)-derived hair cell-like cells

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