ATAC-seq analysis of transgenic human cancer cell line expressing subunits of meiotic cohesin complexes (RAD21L mei-Cohesin)

ABSTRACT: The goal of the study was to investigate the epigenomic properties of somatic cells in response to the induction of mei-cohesin subunits using Tet-on inducible transgenes activated by an rtTA transgene and doxycicline. The study involved applying ATAC-seq (Assay for Transposase-Accessible Chromatin with high-throughput sequencing) analysis of stable transgenic human cancer cell line DLD-1, which expresed Tet-inducible combinations of mei-cohesin subunits. The ATAC-seq method enables mapping DNA accessibility in chromatin with hyperactive Tn5 transposase inserting specific adapters into genome.

ORGANISM(S): Homo sapiens

PROVIDER: GSE201683 | GEO | 2022/09/30

REPOSITORIES: GEO