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Analysis of the Role of NuMA (TDIF) in DNA repair Through Next Generation Sequencing Techniques [OGAP-seq SH-SY5Y]

ABSTRACT: Purpose: To assess the role of NuMA (TDIF) in DNA repair Methods: OGAP-seq was carried out in in each pairwise combination of untreated/hydrogen peroxide treated and no doxycyclin (NuMA (TDIF) WT)/doxycyclin treated (NuMA (TDIF) KD) SH-SY5Y cells in duplicate. The data was mapped using bwa mem and subsequently used to generate bigwigs showing fold-change relative to inputs using MACS2 and various meta-feature profiles using the CGAT suite of bioinformatics tools Results: The data revealed elevated OGAP-seq signal in promoter and enhancer regions upon NuMA (TDIF) KD Conclusions: Our study indicates that NuMA (TDIF) plays a role in the protection from and response to oxidative damage. This protective effect appears to be particularly strong at promoter and enhancer regions.

ORGANISM(S): Homo sapiens

PROVIDER: GSE201981 | GEO | 2022/07/15

REPOSITORIES: GEO

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