Project description:47,880 probe-sets were screened, in the first part of this study, they were analysed for correlation of transcripts levels with body composition traits
Project description:47,880 probe-sets were screened, in the first part of this study, they were analysed for differential expression between two groups differing in coping behavior associated haplotype
Project description:47,880 probe-sets were screened, in the first part of this study, they were analysed for differential expression between two groups differing in coping behavior associated haplotype
Project description:47,880 probe-sets were screened, in the first part of this study, they were analysed for differential expression between different breeds (Duroc and Pietrain) at adult stage.
Project description:47,880 probe-sets were screened, in the first part of this study, they were analysed for differential expression between two groups differing in coping behavior associated haplotype
Project description:47,880 probe-sets were screened, in the first part of this study, they were analysed for differential expression between two groups differing in coping behavior associated haplotype
Project description:47,880 probe-sets were screened, in the first part of this study, they were analysed for differential expression between different breeds (Duroc and Pietrain) at adult stage. RNAs of skeletal muscle from Duroc and Pietrain at adult stage were hybridized to Affymetrix Porcine Snowball Array.
Project description:Temperature stress is one of the main environmental stressors affecting the welfare, health and productivity of livestock. Temperature changes can modify cell membrane components, disrupting the crosstalk between the cell and its surroundings by affecting signaling pathways including Wnt signaling pathway, which subsequently disrupts cell energy metabolism. The present study aims to understand the effect of temperature stress on the expression of genes involved in Wnt signaling pathways, and their interaction with energy metabolism in C2C12 myoblasts cells. The C2C12 cells were exposed to cold stress (35 °C), mild heat stress (39 °C) and severe heat stress (41 °C), whereas 37 °C was used as control temperature. Transcript levels of important genes involved in Wnt signaling including Axin2, Tnks2, Sfrp1, Dkk1, Dact1, Cby1, Wnt5a, Wnt7a, Wnt11, Porcn, Ror2, Daam1, and Ppp3ca were significantly altered under severe heat stress (41 °C), whereas eight Wnt signaling-related transcripts (Daam1, Ppp3ca, Fzd7, Wnt5a, Porcn, Tnks2, Lrp6, and Aes) were significantly altered under cold stress (35 °C) compared to control. Under heat stress transcripts of the Wnt/β-catenin inhibitors (Sfrp1, Dkk1, and Cby1) and negative regulators (Dact1 and Axin2) are activated. A positive correlation between oxidative phosphorylation and Wnt-related transcripts was found under high temperatures. Transcripts of the cell membrane receptors, including Lrp6 and Fzd7, and the members of Wnt/Ca+2 signaling pathway, including Ppp3ca and Porcn were downregulated under cold stress. Many Wnt signaling-related transcripts were positively correlated with glycolysis under cold stress. These findings indicate a cross-talk between Wnt signaling and energy metabolism under thermal stress.
