Transcriptomics

Dataset Information

0

M6A Modification Regulates Planarian Regeneration [scRNA-seq]


ABSTRACT: Regeneration is the regrowth of damaged tissues or organs, a vital mechanism in response to damages from primitive organisms to higher mammals. Planarian possesses active whole-body regenerative capability owning to its vast reservoir of adult stem cells, neoblasts, providing an ideal model to delineate the underlying mechanisms for regeneration. RNA N6-methyladenosine (m6A) modification participates in many biological processes, including stem cell self-renewal and differentiation, in particular the regeneration of hematopoietic stem cells and axons. However, how m6A controls regeneration at the whole-organism level remains largely unknown. Here, we demonstrate that the depletion of m6A methyltransferase regulatory subunit wtap abolishes planarian regeneration, potentially through regulating genes related to cell-cell communication and cell cycle. Single-cell RNA-seq (scRNA-seq) analysis unveils that the wtap knockdown induces a unique type of neural progenitor-like cells (NP-like cells), characterized by specific expression of the cell-cell communication ligand grn. Intriguingly, the depletion of m6A-modified transcripts grn/cdk9 (or cdk7) axis rescues the defective regeneration of planarian caused by wtap knockdown. Overall, our study reveals an indispensable role of m6A modification in regulating whole-organism regeneration.

ORGANISM(S): Schmidtea mediterranea

PROVIDER: GSE202928 | GEO | 2023/04/28

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2023-04-28 | GSE171249 | GEO
2023-04-28 | GSE171247 | GEO
2023-09-29 | GSE216410 | GEO
2023-09-28 | GSE216412 | GEO
2023-08-18 | PXD036899 | Pride
2009-06-30 | E-GEOD-16159 | biostudies-arrayexpress
2014-07-18 | E-GEOD-55575 | biostudies-arrayexpress
2022-09-28 | GSE214225 | GEO
2014-07-18 | E-GEOD-55572 | biostudies-arrayexpress
2016-03-24 | E-GEOD-79523 | biostudies-arrayexpress