Transcriptomics

Dataset Information

0

Topical, immunomodulatory epoxy-tiglianes induce biofilm disruption and healing in acute and chronic skin wounds [HDFa]


ABSTRACT: The management of antibiotic-resistant, bacterial biofilm infections in chronic skin wounds is a significant clinical challenge. Despite advances in diagnosis, many patients do not derive benefit from current anti-infective/antibiotic therapies. Here we report a novel class of epoxy-tiglianes and demonstrate their antimicrobial activity (modifying bacterial growth and inducing biofilm disruption), with structure/activity relationships established against important human pathogens. In vitro, the lead candidate, EBC-1013 stimulated PKC-dependent neutrophil ROS induction and NETosis, and increased expression of wound healing associated cytokines, chemokines and antimicrobial peptides in keratinocytes and fibroblasts. In vivo, topical EBC-1013 induced rapid resolution of infection with increased matrix remodelling in acute thermal injuries. In chronically-infected, diabetic wounds, treatment induced cytokine/chemokine production, inflammatory cell recruitment and complete healing (in 6/7 wounds) with ordered keratinocyte differentiation. These results highlight a non-antibiotic approach involving contrasting, orthogonal mechanisms of action: targeted biofilm disruption and innate immune induction in the treatment of chronic wounds.

ORGANISM(S): Homo sapiens

PROVIDER: GSE202952 | GEO | 2022/05/14

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2022-05-12 | GSE202794 | GEO
2019-08-01 | GSE122297 | GEO
2014-01-01 | E-GEOD-51167 | biostudies-arrayexpress
2014-01-01 | GSE51167 | GEO
| PRJNA837872 | ENA
| PRJNA837275 | ENA
| PRJNA837880 | ENA
2015-11-24 | E-GEOD-75359 | biostudies-arrayexpress
| phs001260 | dbGaP
2024-03-30 | GSE261418 | GEO