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The storage conditions of high fat diet are the key factors for diet induced obesity and liver damage [HFDfre vs. HFDfri]

ABSTRACT: The diet induced obesity (DIO) mouse model has been widely used for obesity studies. Comparing to the composition of nutrients in the diet, the effects of storage conditions for high fat diet (HFD) on metabolic homeostasis have not been systemically investigated. In the current study, we tested the effects of HFD stored in different conditions and found the mice fed by HFD stored in the fridge (HFDfri) gained less weight than freezer (HFDfre). Further analysis revealed the changes of the relative abundance of medium chain triglyceride (MCT) in the HFDfri, which has much lower intestinal absorption rates, contributed to the body weight differences. In contrast, advanced liver damages and elevation of unfolded protein responses (UPR) was observed in the mice fed by the HFDfri. Depletion of UPR regulated gene Nnmt alleviated liver damages via inhibition of integrated stress response (ISR). Our study provided the initial evidence that HFD storage conditions have great impacts on both body weight changes and liver damages in the DIO model.

ORGANISM(S): Mus musculus

PROVIDER: GSE203039 | GEO | 2022/12/31

REPOSITORIES: GEO

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Project description:To profile the expression of circulating miRNAs in a mouse model of diet-induced obesity (DIO) with subsequent weight-reduction with low-fat diet (LFD), eighteen C57BL/6 male mice were grouped into three subgroups as: (1) Control: the mice fed with the standard AIN-76A (fat: 11.5 kcal%) diet for 12 wks; (2) DIO: the mice fed with 58 kcal% high-fat diet for 12 wks; (3) DIO+LFD: the mice fed with high-fat diet for 8 wks to induce obesity, then changed to 10.5 kcal% low-fat diet for subsequent 4 wks. C57BL/6 mice were purchased from BioLasco (Taipei, Taiwan). All housing conditions were maintained, and surgical procedures, including analgesia, were performed in an Association for Assessment and Accreditation of Laboratory Animal Care International (AAALAC)-accredited SPF facility according to national and institutional guidelines. In this experiment, eighteen C57BL/6 wild type male mice were randomly grouped into three subgroups (n=6 in each group): (1) Control: the control mice were fed ad libitum a standard AIN-76A (fat: 11.5 kcal%) diet for 12 wks; (2) DIO: the mice were fed ad libitum a 58 kcal% HFD (D12331; Research Diets Inc., New Brunswick, NJ) for 12 wks to induce obesity; (3) DIO+LFD: the mice fed ad libitum a 58 kcal% HFD (D12331) for 8 wks to induce obesity, then continued the feeding of 10.5 kcal% LFD (D 12329; Research Diets Inc.) for additional 4 wks. Weight measurements were performed on a weekly basis to for these three groups of mice. Evaluation of blood glucose levels was performed at the beginning and in the end of the experiment to confirm that the HFD-fed mice developed an obese and insulin resistant phenotype. After the end of experiment at 12w, all mice were killed. The abdominal WAT of each mice was removed and weighted. Paraffin-embedded abdominal WAT was sectioned at 5 M-NM-<m and stained with hematoxylin and eosin to measure mean adipocyte area. A volume of 1 mL of whole blood was collected into a plain tube and allowed to clot for 1 hour. The sera samples were aliquoted after centrifugation at 3,000 M-CM-^W g for 10 minutes and stored at M-bM-^HM-^R80M-BM-0C until further analysis.

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The storage conditions of high fat diet are the key factors for diet induced obesity and liver damage [HFDfre vs. HFDfri]

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