CGH reveals a neo-X chromosome and biased gene movement in stalk-eyed flies (genus Teleopsis)
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ABSTRACT: In this study, we utilized Comparative Genomic Hybridization (CGH), using probes designed from EST sequence, to identify genes located on the X chromosome of four species in the stalk-eyed fly genus Teleopsis. Analysis of log ratio values from the CGH microarrays for over 3400 genes produces a strongly bimodal distribution that clearly differentiates autosomal from X-linked genes for all four species. Genetic mapping of 35 of these genes in T. dalmanni indicates the CGH results correctly identified chromosomal location in all cases. Syntenic comparison with Drosophila indicates that 90% of the X-linked genes in Teleopsis are homologous to genes located on chromosome 2L in D. melanogaster, suggesting the formation of a nearly complete neo-X chromosome from Muller element B in the Dipteran lineage leading to Teleopsis. Overall, this study demonstrates CGH is a useful technique for identifying chromosomal sex-linkage and should be applicable to other organisms with EST or partial genomic information.
ORGANISM(S): Teleopsis quinqueguttata Teleopsis thaii Teleopsis whitei Teleopsis dalmanni
PROVIDER: GSE20315 | GEO | 2010/03/01
SECONDARY ACCESSION(S): PRJNA125359
REPOSITORIES: GEO
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