Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Droplet-based single-cell RNA-sequencing facilitates transcription analysis of small intestine epithelial cells from SubAA273IEC and littermate control

ABSTRACT: We performed theDroplet-based single-cell RNA-sequencing on small intestine epithelial cells from SubAA273IEC and littermate control and found the effect of ER stress in epithelia cells on epithelial cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE203538 | GEO | 2023/03/13

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Droplet-based single-cell RNA-sequencing facilitates trancritption analysis of small intestine CD4 cells from Germline free Xbp1ΔIEC and Xbp1fl/fl

Project description:We performed theDroplet-based single-cell RNA-sequencing on small intestine CD4 cells from Germline free(GF) Xbp1ΔIEC and Xbp1fl/fl mice and found the effect of ER stress in epithelia cells on CD4 cells.

2023-03-13 | GSE168947 | GEO

Ileal gene expression in Duoxa-/- mice and cohoused littermate controls

2015-06-10 | GSE60933 | GEO

Transcription profiling of mice with transgenic expression of the transcription factor Klf5 in esophageal epithelia compared to littermate controls

Project description:Three mice with transgenic expression of the transcription factor Klf5 in esophageal epithelia were compared with three littermate controls at 3 months of age

2007-04-11 | E-MEXP-923 | biostudies-arrayexpress

Next Generation Sequencing Facilitates analysis of IL17 EGFP positive and negative cells from small intestine (SI) lamina propia (lp) genes expression

Project description:We performed the bulk RNA seq on IL17 EGFP positive and negative cells from small intestine (SI) lamina propia (lp) of antibiotic treated XBP1ΔIEC mice and found the different genes expression in the setting of IEC ER stress induced Th17 positive and negative cells

2023-03-13 | GSE203537 | GEO

The Heterochromatin protein 1 is a regulator in RNA splicing precision deficient in ulcerative colitis

Project description:Total RNA was extracted from mouse Villin-creERT2:Cbx3-/- mice epithelial cells from the small intestine crypt, villi and colon epithelia

2022-10-24 | GSE192800 | GEO

Ileal gene expression in Duoxa-/- mice and cohoused littermate controls

Project description:Dual oxidases play a role in innate host defense at barrier epithelia. We examined the effect of loss of dual oxidase function (duoxa-/-) on gene expression in the mouse terminal ileum at homeostasis. To control for cage/litter effects, duoxa-/- were cohoused with wild type littermate controls. Ileal mucosal samples were obtained from duoxa-/- mice and cohoused wild type littermates from three separate cages (litters). For each cage, RNA was pooled from n=2-3 animals per genotype.

2015-06-10 | E-GEOD-60933 | biostudies-arrayexpress

Distinct human stem cell populations in small and large intestine

Project description:The intestine is composed of an epithelial layer, containing rapidly proliferating cells that mature into two distinct anatomic regions, the small and the large intestine. Although previous studies have identified stem cells as the cell-of-origin for the whole intestine, no studies have compared stem cells derived from the small and large intestine. Here, we report intrinsic differences between these two populations of cells. Primary epithelial cells isolated from human fetal small and large intestine and expanded with Wnt agonist, R-spondin 2, displayed differential expression of stem cell markers and separate hierarchical clustering of gene expression involved in differentiation, proliferation and disease pathways. Using a three-dimensional in vitro differentiation assay, single cells derived from small and large intestine formed distinct organoid architecture with cellular hierarchy similar to that found in primary tissue. Our characterization of human fetal intestinal stem cells defies the classical definition proposed by most where small and large intestine are repopulated by an identical epithelial stem cell and raises the question of the importance of intrinsic and extrinsic cues in the development of intestinal diseases. 12 samples were analyzed. They consisted of human fetal small and large intestine (SI; n=6 and LI; n=6) stem cells, expanded with Wnt agonist and R-spondin 2. Differential expression of genes in epithelial cells from both the large and small intestine were observed.

2015-01-15 | E-GEOD-56525 | biostudies-arrayexpress

Epithelial cell diversity in small intestine of pigs

Project description:To establish better understanding of specific epithelial cells found across different regions of the small intestine in pigs, we utilized single-cell RNA sequencing (scRNA-seq) to recover and analyze epithelial cells from duodenum, jejunum, and ileum. Cells identified included crypt cells, enterocytes, BEST4 enterocytes, goblet cells, and enteroendocrine (EE) cells. Overall, results provide new information on regional localization and transcriptional profiles of epithelial cells in the pig small intestine.

2023-01-18 | GSE208613 | GEO

Distinct human stem cell populations in small and large intestine

2015-01-15 | GSE56525 | GEO