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M6a methylation drives IMP1 regulation during human neuronal differentiation.

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Homo sapiens

PROVIDER: GSE203571 | GEO | 2024/04/13

REPOSITORIES: GEO

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m6a methylation drives IMP1 regulation during human neuronal differentiation [miCLIP]

Project description:Human neurodevelopment requires differentiating neurons to establish large networks of connections in a highly stereotyped manner. Neuronal differentiation in particular requires RNA-binding proteins to spatiotemporally regulate thousands of different mRNAs. Yet how these proteins precisely relate to neuronal development and coordinate the expression of functionally coherent genes in a cell type specific manner is only partially understood. To address this, we sought to clarify how the paradigmatic RNA-binding protein IMP1/IGF2BP1, an essential developmental factor, selects and regulates its RNA targets transcriptome-wide during the differentiation of human pluripotent stem cell-derived neural precursor cells to their neuronal counterparts. We used a combination of systemic and molecular analyses to show that IMP1 directly binds to and regulates the expression of a large sets of mRNAs.

2024-04-13 | GSE203570 | GEO

m6a methylation drives IMP1 regulation during human neuronal differentiation [iCLiP]

2024-04-13 | GSE203569 | GEO

m6a methylation orchestrates IMP1 regulation of microtubules during human neuronal differentiation

Project description:Human neurodevelopment requires differentiating neurons to establish large networks of connections in a highly stereotyped manner. Neuronal differentiation in particular, requires RNA-binding proteins to spatiotemporally regulate thousands of different mRNAs. Yet, how these proteins precisely relate to neuronal development and coordinate the expression of functionally coherent genes in a cell type specific manner is only partially understood. To address this, we sought to understand how the paradigmatic RNA-binding protein IMP1/IGF2BP1, an essential developmental factor, selects and regulates its RNA targets transcriptome-wide during the differentiation of human neurons. We used a combination of systemic and molecular analyses to show that IMP1 directly binds to and regulates the expression of a large set of mRNAs that govern microtubule assembly, an essential process and a key driver of neuronal differentiation. We also show that m6A methylation during the transition from neural precursors to neurons drives both the selection of IMP1 mRNA targets and their translation potential. Our findings establish m6A methylation as a key mechanism coordinating the regulatory action of IMP1 on human neuronal architecture.

2024-06-10 | PXD034341 | Pride

m6a methylation drives IMP1 regulation during human neuronal differentiation [RNA-seq]

2024-04-13 | GSE203568 | GEO

m6a methylation drives IMP1 regulation during human neuronal differentiation.

Project description:m6a methylation drives IMP1 regulation during human neuronal differentiation.

| PRJNA841463 | ENA

m6a methylation drives IMP1 regulation during human neuronal differentiation [miCLIP]

Project description:m6a methylation drives IMP1 regulation during human neuronal differentiation [miCLIP]

| PRJNA841466 | ENA

m6a methylation drives IMP1 regulation during human neuronal differentiation [iCLiP]

Project description:m6a methylation drives IMP1 regulation during human neuronal differentiation [iCLiP]

| PRJNA841467 | ENA

m6a methylation drives IMP1 regulation during human neuronal differentiation [RNA-seq]

Project description:m6a methylation drives IMP1 regulation during human neuronal differentiation [RNA-seq]

| PRJNA841468 | ENA

m6A mRNA Methylation Regulates Early Pancreatic Cell Differentiation

Project description:N6-methyladenosine (m6A) is the most abundant chemical modification in mRNA and plays important roles in human and mouse embryonic stem cell pluripotency, maintenance, and differentiation. We have recently reported, for the first time, the role of m6A in the postnatal control of β-cell function in physiological states and in Type 1 and 2 Diabetes. However, the precise mechanisms by which m6A acts to regulate the development of human and mouse pancreas are unexplored. Here, we show that the m6A landscape is dynamic during human pancreas development, and that METTL14, one of the m6A writer complex proteins, is essential for the early differentiation of both human and mouse pancreatic cells.

| S-SCDT-10_1038-S44318-024-00213-2 | biostudies-other

Negative regulation of Yap during neuronal differentiation.

Project description:Regulated proliferation and cell cycle exit are essential aspects of neurogenesis. The Yap transcriptional coactivator controls proliferation in a variety of tissues during development, and this activity is negatively regulated by kinases in the Hippo signaling pathway. We find that Yap is expressed in mitotic mouse retinal progenitors and it is downregulated during neuronal differentiation. Forced expression of Yap prolongs proliferation in the postnatal mouse retina, whereas inhibition of Yap by RNA interference (RNAi) decreases proliferation and increases differentiation. We show Yap is subject to post-translational inhibition in the retina, and also downregulated at the level of mRNA expression. Using a cell culture model, we find that expression of the proneural basic helix-loop-helix (bHLH) transcription factors Neurog2 or Ascl1 downregulates Yap mRNA levels, and simultaneously inhibits Yap protein via activation of the Lats1 and/or Lats2 kinases. Conversely, overexpression of Yap prevents proneural bHLH proteins from initiating cell cycle exit. We propose that mutual inhibition between proneural bHLH proteins and Yap is an important regulator of proliferation and cell cycle exit during mammalian neurogenesis.

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