Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Single-cell transcriptomic profiling of ~1500 K562 cells expressing dCas9-KRAB to compare Illumina and Ultima sequencing platforms.

ABSTRACT: ~1500 K562 cells expressing dCas9-KRAB were profiled on the 10x Genomics 3' v3 droplet-based scRNA-seq platform. The resulting library was sequenced on the Illumina and Ultima sequencing platforms.

ORGANISM(S): Homo sapiens

PROVIDER: GSE204750 | GEO | 2022/05/25

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Single-cell transcriptomic profiling of ~1500 K562 cells expressing dCas9-KRAB to compare Illumina and Ultima sequencing platforms.

Project description:Single-cell transcriptomic profiling of ~1500 K562 cells expressing dCas9-KRAB to compare Illumina and Ultima sequencing platforms.

| PRJNA842077 | ENA

Generation and characterization of inducible KRAB-dCas9 iPSCs from primates for cross-species CRISPRi

Project description:Comparisons of molecular phenotypes across primates provide unique information to understand human biology and evolution and single-cell RNA-seq CRISPR interference screens are a powerful approach to analyze them. Here, we generate and validate three human, three gorilla and two cynomolgus iPS cell lines that carry a dox-inducible KRAB-dCas9 construct in the AAVS1 locus. We show that despite variable expression levels of KRAB-dCas9 among lines, comparable downregulation of target genes and comparable phenotypic effects are observed in a single-cell RNA-seq CRISPR interference screen. Hence, we provide valuable resources for performing and further extending CRISPRi screens in human and non-human primates.

2024-03-26 | GSE241293 | GEO

Generation and characterization of inducible KRAB-dCas9 iPSCs from primates for cross-species CRISPRi

2024-02-20 | GSE255980 | GEO

Synthetic epigenetic reprogramming of mesenchymal to epithelial states using the CRISPR/dCas9 platform in triple negative breast cancer [methylation array]

Project description:Infinium MethylationEPIC (850K) BeadChip data for wildtype SUM159 cells (2 replicates), SUM159 cells transfected with plV-KRAB (dCas9 CRISPRi system; 3 replicates), and SUM159 cells transfected with dCas9-KRAB and 4 targeting gRNAs against the ZEB1 promoter (3 replicates)

2023-04-17 | GSE210273 | GEO

Generation and characterization of inducible KRAB-dCas9 iPSCs from primates for cross-species CRISPRi

Project description:Generation and characterization of inducible KRAB-dCas9 iPSCs from primates for cross-species CRISPRi

| PRJNA1007648 | ENA

Generation and characterization of inducible KRAB-dCas9 iPSCs from primates for cross-species CRISPRi

Project description:Generation and characterization of inducible KRAB-dCas9 iPSCs from primates for cross-species CRISPRi

| PRJNA1077348 | ENA

Inhibition of uPA expression by CRISPR-dCas9 DNA methyltransferases

Project description:We report the generation of CRISPR-dCas9 DNA methyltransferases to mediate targeted DNA methylation. Using the dCas9-BFP-DNMT3A and dCas9-BFP-DNMT3B methyltransferases, we have demonstrated that these two methyltransferase can mediate targeted methylation in three human genes tested: uPA, TGFBR3, and CDKN2A in human HEK293T cells. We also showed that these methyltransferases could mediate gene inhibition. five samples co-transfected with five uPA sgRNAs and each of the four dCas9 fusions, or control transfection with pUC19 plasmid

2016-06-01 | E-GEOD-74935 | biostudies-arrayexpress

Identification of a novel enhancer of CEBPE essential for granulocytic differentiation

Project description:RNA-Seq of Control, dCas9-KRAB-EV, dCas9-KRAB-sg2 and dCas9-KRAB-sg5

2022-05-20 | GSE122676 | GEO

Single-cell profiling of cell cultures during endoMT induction.

Project description:Single-cell RNA sequencing (scRNAseq) data from EndoMT Day 1 dcas9-KRAB-MeCP2 (KRAB Control Day1), EndoMT Day 3 dcas9-KRAB-MeCP2 (KRAB Control Day3), and EndoMT Day 3 dcas9-KRAB-MeCP2 + rs117430032 gRNA (rs117430032i) cells.

2022-12-16 | GSE193206 | GEO

RNA-seq following enhancer repression

Project description:Using RNA-seq to identify genes regulated by dCas9-KRAB mediated enhancer repression in NCI-H2009 lung adenocarcinoma cells NCI-H2009 cells were first transfected with dCas9-KRAB fusion and subsequently transfected with no guide RNA (Empty), a guide RNA that has no target in the human genome (Dummy), and two separate guide RNAs (e3 #1 and e3 #2) recognizing the enhancer region of our study.

2015-10-26 | E-GEOD-72001 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data