Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptomic changes from resulting from DARPP-32 modulation in breast cancer

ABSTRACT: DARPP-32 expression was reduced in oestrogen receptor positive luminal B breast cancer cells (T47D) using siRNA and control and DARPP-32 knockdown cells were subject to stimulation with estradiol17β (E2) or inhibition of protein kinase A (PKA) with PKA inhibitor fragment (6-22) amide prior to RNA sequencing. A bioinformatics pipeline of Trim Galore, Kallisto, DESeq2 and Qiagen Ingenuity Pathway Analysis was used for pathway analysis.

ORGANISM(S): Homo sapiens

PROVIDER: GSE204836 | GEO | 2023/10/30

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

DARPP32, a target of hyperactive mTORC1 in the retinal pigment epithelium

Project description:The mechanistic target of rapamycin (mTOR) is assembled into signaling complexes of mTORC1 or mTORC2, and plays key roles in cell metabolism, stress response, nutrient and growth factor sensing. Accumulating evidence from human and animal model studies has demonstrated a pathogenic role of hyperactive mTORC1 in age-related macular degeneration (AMD). The retinal pigment epithelium (RPE) is a primary injury site in AMD. In mouse models of RPE-specific deletion of Tuberous sclerosis 1 (Tsc1), which encodes an upstream suppressor of mTORC1, the hyperactivated mTORC1 metabolically reprogrammed the RPE and led to the degeneration of the outer retina and choroid (CH). In the current study, we used single-cell RNA sequencing (scRNA-seq) to identify a novel RPE mTORC1 downstream protein, dopamine and cyclic AMP-regulated phosphoprotein of molecular weight 32,000 (DARPP-32). DARPP-32 was not found in healthy RPE but localized to drusen and basal linear deposits in human AMD eyes. In animal models, overexpressing DARPP-32 by adeno-associated virus (AAV) led to abnormal RPE structure and function. The data indicate that DARPP-32 is a previously unidentified signaling protein subjected to mTORC1 regulation and may contribute to RPE degeneration in AMD.

2022-07-29 | GSE209908 | GEO

Xylella fastidiosa 32 strain:32

Project description:Xylella fastidiosa 32 strain:32 Genome sequencing and assembly

| PRJNA171052 | ENA

16-32-00914

Project description:16-32-00914 Map

| PRJNA446039 | ENA

Sequencing Caenorhabditis sp. 32

Project description:Sequencing Caenorhabditis sp. 32

| PRJEB12601 | ENA

Sinorhizobium meliloti strain:32

Project description:Sinorhizobium meliloti 32 Resequencing

| PRJNA501431 | ENA

Yersinia pseudotuberculosis str. 32

Project description:Yersinia pseudotuberculosis str. 32

| PRJNA46835 | ENA

Bradyrhizobium japonicum strain:USDA 32 | isolate:USDA 32

Project description:Bradyrhizobium japonicum USDA 32 genome sequencing

| PRJNA708386 | ENA

Bacteroides faecis CAG:32

Project description:Bacteroides faecis CAG:32 overview

| PRJNA207251 | ENA

Histone H2B^3-32

Project description:Histone H2B was mutated to give H2B^3-32. Total RNA from three replicate cultures of wild-type and mutant was isolated and the expression profiles were determined using Affymetrix arrays. Comparisons between the sample groups allow the identification of genes regulated by H2B N-terminal domain. Keywords: repeat

2006-04-08 | GSE3802 | GEO

RNAseq in IMR-32 neuroblastoma cells

Project description:IMR-32 cells were subjected to lentiviral YRNA infection or nELAVL RNAi and/or UV stress followed by RNAseq analysis to monitor RNA level changes

2016-02-17 | GSE53698 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data