Transcriptomic analysis of the effect of Kap120 deletion
Ontology highlight
ABSTRACT: The effect of Kap120 deletion at transcriptional level was investigated by GRO analysis of the transcription rates (TR) and mRNA levels (RA) genome-wide. mRNA stabilities can be calculated as RA /TR for each gene in all samples.
Project description:We study the transcriptional response of wt S. cerevisiae cells to alkaline pH by using a genome-wide method (Genomic run-on) that allows for the determination of transcription rates (TR) and mRNA amounts (RA) for each gene. The stabilities of the mRNAs can be calculated from the TR and RA data.
Project description:The effect of Sfp1 depeltion at transcriptional level was investigated by construting a AID-Sfp1 strain (Auxin sensitive degron) that allowed to shutoff the Sfp1 expression and analyze the primary effects on synthesis rates (SR) and mRNA levels (RA) genome-wide. mRNA stabilities can be calculated as RA /SR for each gene in all samples.
Project description:We have taken five different time points of a Yeast culture in exponential grow in rich medium, each one elapsed ten minutes from de previous one (from OD600 0.36 to 0.47). Then, for each time point we have measured the transcription rates (TR) and mRNA amounts (RA) for all the genes using the Genomic run-on (GRO) technique .
Project description:We have taken five different time points of a Yeast culture in exponential grow in rich medium, each one elapsed ten minutes from de previous one (from OD600 0.36 to 0.47). Then, for each time point we have measured the transcription rates (TR) and mRNA amounts (RA) for all the genes using the Genomic run-on (GRO) technique . For each time point we have measured the transcription rates (GRO) with 3 replicates (except for the t2 that only has 2 replicates due to technical problems) and mRNA amounts (cDNA RP) with 3 replicates for all the genes. The data were normalized using a gDNA hybridization for each filter.
Project description:The goal of the project was to study the effects on transctiption and mRNA stability after iron depletion. We analyzed the effect of iron depletion caused by 100 μM of the Fe2+-specific chelator bathophenanthroline disulfonic acid (BPS) on the transcription rates, mRNA stabilities and mRNA levels by doing Genomic Run-On (GRO) experiments at 0, 10, 30, 90, 180 and 360 minutes after iron limitation.
Project description:The goal of the project was to study the effects on transcription and mRNA stability of the Xrn1 sudden depletion. We analyzed the effect of Xrn1 depletion caused by protein degradation of an Auxin-degron fusion on the transcription rates, mRNA stabilities and mRNA levels by doing Genomic Run-On (GRO) experiments at 30 min after Auxin addition with a control at 0 min.
Project description:We analyzed the effect of SPT16 on the transcription rates, mRNA stabilities and mRNA levels by doing GRO experiments in a thermosensitive spt16 point mutant (G132D) mutant at 35 ºC comparing with permisive temperature (30 ºC) and a wt treated in the same way.
Project description:The goal of the project was to study synthesis rates (SR) and mRNA levels (RA) genome wide in a series of aneuploid strains to check for the posible variation in SR and RA in the genes of the aneuploid chromosome withe regard to the rest of the genome. We used Genomic Run-On (GRO) experiment to mesaure SR and RA values.
Project description:Within a GRO experiment, samples for mRNA amount (RA) were taken at 0, 4, 11, 16, 26 and 40 minutes after heat stress (from 25ºC to 37ºC). Analysis was done in filters also used for Transcription rate (TR) analysis of aliquots from the same cultures.
Project description:We analyzed the effect of PUB1 and NGR1 on the transcription rates, mRNA stabilities and mRNA levels by doing GRO experiments in a deletion mutants comparing with a wild type in both glucose (YPD) and galactose (YPGal) media. Some data for the wt in YPGal are not included because were already in GEO database: GSE1002 accession number.