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Effects of Mitochondrial Disturbances on Transcriptional Profile of Arabidopsis ucp double mutant plants

ABSTRACT: Three genes encoding mitochondrial uncoupling proteins (UCPs) have been described in Arabidopsis thaliana (UCP1 to UCP3). In plants, they are characterized as regulators of oxidative stress, and, for instance, much of the biological data were obtained for the UCP1 and UCP2 isoforms compared with UCP3. To gain further insights, we investigated the transcriptome profiles of ucp1 ucp3 double mutant plants during vegetative phase. We found that the double knockdown of UCP1 and UCP3 promotes important transcriptional reprogramming with clear alterations in the expression of genes related to mitochondrial and chloroplast function as well as those responsive to abiotic stress, suggesting that profound disturbances occur throughout the cell. These findings support a better elucidation for effects of UCP genes and provide insights into the role of UCP3 isoform together with UCP1 in the entire plant.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE205194 | GEO | 2023/05/01

REPOSITORIES: GEO

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Project description:Recent studies on mouse and human skeletal muscle (SM) demonstrated the important link between mitochondrial function and the cellular metabolic adaptation. To identify key compensatory molecular mechanisms in response to chronic mitochondrial distress, we analyzed mice with ectopic SM respiratory uncoupling in uncoupling protein 1 transgenic (UCP1-TG) mice as model of muscle-specific compromised mitochondrial function. Here we describe a detailed metabolic reprogramming profile associated with mitochondrial perturbations in SM, triggering an increased protein turnover and amino acid metabolism with induced biosynthetic serine/1-carbon/glycine pathway and the longevity-promoting polyamine spermidine as well as the trans-sulfuration pathway. This is related to an induction of NADPH-generating pathways and glutathione metabolism as an adaptive mitohormetic response and defense against increased oxidative stress. Strikingly, consistent muscle retrograde signaling profiles were observed in acute stress states such as muscle cell starvation and lipid overload, muscle regeneration, and heart muscle inflammation, but not in response to exercise. We provide conclusive evidence for a key compensatory stress-signaling network that preserves cellular function, oxidative stress tolerance, and survival during conditions of increased SM mitochondrial distress, a metabolic reprogramming profile so far only demonstrated for cancer cells and heart muscle.-Ost, M., Keipert, S., van Schothorst, E. M., Donner, V., van der Stelt, I., Kipp, A. P., Petzke, K.-J., Jove, M., Pamplona, R., Portero-Otin, M., Keijer, J., and Klaus, S. Muscle mitohormesis promotes cellular survival via serine/glycine pathway flux. Control C57BL/6J wildtype male mice, aged 12 weeks, are compared to UCP1 transgene littermates fed purified low fat diet (BIOCLAIMS, Hoevenaars et al., Genes and Nutrition 2012) for 12 weeks . At the end, mice were sacrificed, gastrocnemius skeletal muscle was immediately dissected and snap frozen in liquid nitrogen. Total RNA was isolated, quantified and qualified, and subsequently used for global gene expression profiling using Agilent 8x60K microarrays. In total, 6 arrays of individual WT and 7 arrays of individual UCP1-TG samples were normalized, together with samples from the complete SUPERSERIES.

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Effects of Mitochondrial Disturbances on Transcriptional Profile of Arabidopsis ucp double mutant plants

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