Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression profiles following GR24, smoke water and KAR1 treatments in Nicotiana benthamiana seedlings

ABSTRACT: GR24, a synthetic strigolactone, and KAR1, the main bioactive compound in smoke water, both share a common α,β unsaturated furanone moiety which promotes biomass accumulation in three week old N. benthamiana seedlings. In order to investigate whether this D ring is responsible for the biomass accumulation, gene expression profiles were evaluated for co-expression on the Agilent 44k N. tabacum microarray. GR24, smoke and KAR1 induced different transcripts, and suggests that they trigger independent growth responses.

ORGANISM(S): Nicotiana tabacum Nicotiana benthamiana

PROVIDER: GSE20520 | GEO | 2010/02/25

SECONDARY ACCESSION(S): PRJNA125247

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Gene expression profiles following GR24, smoke water and KAR1 treatments in Nicotiana benthamiana seedlings

Project description:GR24, a synthetic strigolactone, and KAR1, the main bioactive compound in smoke water, both share a common Î±,Î² unsaturated furanone moiety which promotes biomass accumulation in three week old N. benthamiana seedlings. In order to investigate whether this D ring is responsible for the biomass accumulation, gene expression profiles were evaluated for co-expression on the Agilent 44k N. tabacum microarray. GR24, smoke and KAR1 induced different transcripts, and suggests that they trigger independent growth responses. Control (untreated), GR24 (10-7M), smoke water (1:1000 dilution) and butenolide (10-7M) gene expression profiles were evaluated on three week old seedlings, in two independent experimental trials.

2010-04-22 | E-GEOD-20520 | biostudies-arrayexpress

ppmax2 response to gr24-Strigopath/Physcomitrella patens

Project description:4plex_physco_2014-05 - ppmax2 response to gr24 - How does the Ppmax2 moss mutant respond to Strigolactone (GR24)? - Two moss genotypes are used: WT and the Ppmax2 mutant. Moss tissues are fragmented, then plated on medium (Petri dish with cellophane disks) and cultivated for 3 weeks. Moss tissues are then transfered for 6 hours on acetone-containing medium (control treatment, for WT and Ppmax2) or GR24 (1 microM, in acetone)-containing medium (for Ppmax2). After 6 hours, the moss tissues are collected, quickly forzen in liquid nitrogen. RNA are isolated using the Quiagen RNeasy Plant mini kit (including a RNase-free DNase treatment on column). Two similar experiments (T1 and T2) have been led.

2017-04-22 | GSE80578 | GEO

The response of the root proteome to the synthetic strigolactone GR24 in Arabidopsis

Project description:Strigolactones are plant metabolites that act as phytohormones and rhizosphere signals. Whereas most research on unraveling the action mechanisms of strigolactones is focused on plant shoots, we investigated proteome adaptation during strigolactone signaling in the roots of Arabidopsis thaliana. Through large-scale, time-resolved, and quantitative proteomics, the impact of the strigolactone analog rac-GR24 was elucidated on the root proteome of the wild type and the signaling mutant more axillary growth 2 (max2). Our study revealed a clear MAX2-dependent rac-GR24 response: an increase in abundance of enzymes involved in flavonol biosynthesis, which was reduced in the max2-1 mutant. Mass spectrometry-driven metabolite profiling and thin-layer chromatography experiments demonstrated that these changes in protein expression lead to the accumulation of specific flavonols. Moreover, quantitative RT-PCR revealed that the flavonol-related protein expression profile was caused by rac-GR24--induced changes in transcript levels of the corresponding genes. This induction of flavonol production was shown to be activated by the two pure enantiomers that together make up rac-GR24. Finally, our data provide much needed clues concerning the multiple roles played by MAX2 in the roots and a comprehensive view of the rac-GR24--induced response in the root proteome.

2022-02-28 | PXD004269 | Pride

The response of the root proteome to the synthetic strigolactone GR24 in Arabidopsis

2016-06-20 | PXD003879 | Pride

RNA-seq of Arabidopsis seedlings with the treatment of GR24-5DS

Project description:This dataset provides deep-profiling of the Arabidopsis transcriptome of mRNA of 7-day whole seedling on treatment with strigolactone (SL) isomer GR24-5DS. To understand the transcriptome response and genes regulated by SL, we used Columbia (Col0) ecotype. 7-day old seedlings are treated with 2.5uM GR24-5DS for 3hrs and samples are frozen immediately after treatment. 6 samples: 3 biological replicates Control untreated; 3 biological replicates treated with GR24-5DS.

2022-09-20 | E-MTAB-11329 | biostudies-arrayexpress

Plant-derived compounds strigolactone GR24 and pinosylvin modulate gene expression in rat L6 skeletal muscle cells

Project description:We investigated the effects of novel SIRT1 activating plant-derived compounds strigolactone GR24 and pinosylvin on gene expression in rat L6 skeletal muscle myotubes. Resveratrol was included in analyses as a reference compound.

2017-12-18 | GSE90833 | GEO

Tobacco RNA Dependent RNA Polymerase 1 affects the expression of defence related genes in Nicotiana benthamiana during geminivirus infection

Project description:RNA dependent RNA Polymerase 1 of Nicotiana tabacum modulates ToLCV pathogenesis by influencing a number of defence related genes in N. benthamiana plants.

2020-06-03 | GSE144074 | GEO

Transcriptomic Analysis and Gene Expression Patterns of Nicotiana benthamiana in Response to Low Root-Zone Temperatures and Ultraviolet Radiation

Project description:We explored the effects of low temperature (LT) and UV exposure (LT or UV alone and combined) on changes in transcription of N. benthamiana plants. Agilent One-Color Gene Expression Microarray analysis was conducted using an Agilent Tobacco 4 × 44 K format N. tabacum Array.

2021-08-31 | E-MTAB-10871 | biostudies-arrayexpress

Escherichia coli strain:Gr24

Project description:Escherichia coli strain:Gr24 Genome sequencing

| PRJNA1146892 | ENA

Identification of NMD regulated genes in Nicotiana benthamiana

Project description:Nonsense mediated decay (NMD) is a translation termination coupled quality control system. NMD identifies and degrades aberrant mRNAs containing premature termination codons, thereby preventing the accumulation of detrimental truncated proteins. NMD also controls the expression of several normal mRNAs and non-coding transcripts. RNA-seq assays were conducted to identify the NMD regulated genes in Arabidopsis. However, NMD targets have not been studied in other plants. To identify the conserved NMD targets, we wanted to identify the NMD regulated genes in Nicotiana benthamiana model species. Virus induced gene silencing was used to inactivate UPF1 NMD key factor in N. benthamiana, and then comparative RNA-seq experiment was carried out using UPF1-silenced and control–silenced plants. We found that in N. benthamiana significantly more genes are controlled by NMD than in Arabidopsis and that, surprisingly few conserved NMD targets can be recognized. These results suggest that while the mechanism of NMD is highly conserved, the set of NMD controlled genes can be quickly changed in plants.

2019-07-01 | GSE109130 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data