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ChIP-Seq of DNA lesions from irradiated and non-irradiated HaCaT cells

ABSTRACT: This study aims to investigate the binding DNA lesions of AIM2 in HaCaT cells exposed to 0Gy and 20Gy by ChIP-Seq. In this study, HaCaT cells were maintained in DMEM. All culture media were supplemented with 10% fetal bovine serum (FBS; Biological Industries, Kibbutz Beit-Haemek, Israel). Cells were grown at 37 °C in 5% CO2 incubators. Cells were exposed to 20Gy of ionizing radiation using X-ray linear accelerator (Rad Source, Suwanee, GA) at a fixed dose rate of 1.15 Gy/min.Four hours after irradiation, specific antibody against AIM2 was used to pull down AIM2 and the binding DNA lesions, which was then analyzed through ChIP-Seq.

ORGANISM(S): Homo sapiens

PROVIDER: GSE205250 | GEO | 2022/12/31

REPOSITORIES: GEO

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mRNA and lncRNA expression analysis of irradiated and nonirradiated human HaCaT cells

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