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Effect of ectopic expression of EtROP2 kinase on gene expression of avian epithelial cells (Clec213)

ABSTRACT: EtROP2 is a virulence determinant produced by Eimeria tenella in host cells, during and early after cell invasion. We compared transcriptomic analyses of avian cells transfected either by control plasmid or by plasmid allowing EtROP2 expression. We used an avian epithelial cell model developed in our lab (Clec213). Transfected avian cells were FACS sorted by fluorescent marker in order to reduce background noise from non-transfected cells. This analysis allowed us to assess EtROP2 effect on host cell transcription and possibly identify host signalling pathways modified by the parasite. These signalling pathways will pave the way to the identification of potential host protein targeted by parasite virulence factors.

ORGANISM(S): Gallus gallus

PROVIDER: GSE205391 | GEO | 2023/05/26

REPOSITORIES: GEO

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