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Gene expression profile at single cell level for induction of human primordial germ cell-like cells (hPGCLCs) in a biomimetic culture

ABSTRACT: Transcriptome at the single-cell level of a biomimetic culture of primed human pluripotent stem cells (hPSCs) was profiled at Day 3 and Day 8. The biomimetic culture, which mimics the peri-implantation human epiblast development, provides an easily implementable culture protocol for deriving human primordial germ cell-like cells (hPGCLCs) from primed hPSCs .

ORGANISM(S): Homo sapiens

PROVIDER: GSE205611 | GEO | 2022/06/10

REPOSITORIES: GEO

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An ExtendedCulture System that Supports Human Primordial Germ Cell-Like Cells Survival and Initiation of DNA Methylation Erasure

Project description:In this study, we evalauted the transcriptionl profiles of human primordial germ cell-like cell (hPGCLCs) cultured for an extended period on STO feeders in FR10 media for an additional 10 days (D4C10). The hPGCLCs were obtained from two different human embryonic stem cell line (hESCs), UCLA 1 (XX) and UCLA2 (XY) for the RNA sequencing data. For whole genome bisulfite sequencing, we evaluate primed UCLA2 hESCs, directly out of the aggragate hPGCLCs (Day 4= D4) and extended culture hPGCLCs D4C10. Our D4C10 hPGCLCs were compared to prior RNA-sequencing data generated in our laboraty, which revealed that D4C10 hPGCLCs retain germline identy and closely resemble D4 hPGCLCs at the transcriptional level. Our WGBS data revealed that extended culture hPGCLCs at D4C10 have a decrease in CG methlyation, as compared to the starting hESCs and D4 hPGCLCs.

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