Other

Dataset Information

0

The Mediator complex regulates enhancer-promoter interactions [Capture C]


ABSTRACT: Enhancer-mediated gene activation generally requires physical proximity between enhancers and their target gene promoters. However, the molecular mechanisms by which interactions between enhancers and promoters are formed are not well understood. Here, we investigate the function of the Mediator complex in the regulation of enhancer-promoter interactions, by combining rapid protein depletion and high-resolution MNase-based chromosome conformation capture approaches. We show that depletion of Mediator leads to reduced enhancer-promoter interaction frequencies, which are associated with a strong decrease in gene expression. In addition, we find increased interactions between CTCF-binding sites upon Mediator depletion. These changes in chromatin architecture are associated with a re-distribution of the Cohesin complex on chromatin and a reduction in Cohesin occupancy specifically at enhancers. Our results indicate that enhancer-promoter interactions are dependent on an interplay between the Mediator and Cohesin complexes and provide new insights into the molecular mechanisms by which communication between enhancers and promoters is regulated.

ORGANISM(S): Homo sapiens

PROVIDER: GSE205899 | GEO | 2023/04/13

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2023-04-13 | GSE226300 | GEO
2023-04-13 | GSE225294 | GEO
2023-04-13 | GSE205946 | GEO
2023-04-13 | GSE205905 | GEO
2023-04-13 | GSE205947 | GEO
2018-10-19 | GSE115340 | GEO
2015-08-31 | E-GEOD-57436 | biostudies-arrayexpress
2021-09-10 | GSE181694 | GEO
2013-05-22 | E-GEOD-42237 | biostudies-arrayexpress
2013-04-24 | E-GEOD-42397 | biostudies-arrayexpress