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Expression data from Che-1 (AATF) depleted SKBR3 and MDA-468 cell lines

ABSTRACT: Che-1 is a RNA Polymerase II binding protein involved in the regulation of gene transcription. We have observed that Che-1 depletion induces apoptosis in several cancer cells expressing mutated forms of p53. We used microarrays to investigate classes of genes regulated by Che-1 in one of these cell lines.

ORGANISM(S): Homo sapiens

PROVIDER: GSE20622 | GEO | 2010/03/05

SECONDARY ACCESSION(S): PRJNA124809

REPOSITORIES: GEO

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Expression data from Che-1 (AATF) depleted HCT 116 cell line

Project description:Che-1 is a RNA Polymerase II binding protein involved in the regulation of gene transcription. Che-1 emerges as an important adaptor that connects transcriptional regulation, cell-cycle progression, checkpoint control, and apoptosis. We have observed that Che-1 depletion sensitizes cells to chemotherapy. We used microarrays analysis to investigate classes of genes regulated by Che-1. Total RNA was extracted from control and Che-1 depleted HCT 116 cells 48 hours after transient transfection of siRNA.

2013-03-12 | E-GEOD-45009 | biostudies-arrayexpress

Expression data from Che-1 (AATF) depleted HCT 116 cell line

2013-03-12 | GSE45009 | GEO

Che-1/AATF-induced transcriptionally active chromatin promotes cell proliferation in Multiple Myeloma

Project description:We have strong evidence on the essential role of Che-1 protein in promoting the proliferation of MM cells by sustaining chromatin dynamics and cellular transcription on a global scale using numerous experimental approaches, including a generation of a specific transgenic mouse model.

2020-12-08 | GSE149031 | GEO

Che-1/AATF sustains cell growth and global transcription in Multiple Myeloma through the maintenance of open chromatin structure [ChIP-Seq]

Project description:Tumor transformation is the result of genetic modifications that alter gene transcription, thus producing a specific oncogenic program. However, in recent years, several exciting discoveries have shown that also aberrant epigenetic modifications play a major role in the genesis and progression of cancer. Multiple myeloma (MM) is a cancerous pathology resulting from the clonal expansion of plasma cells, and characterized by abnormal production and secretion of antibody proteins. This disease shows a great heterogeneity, caused by both genetic and epigenetic abnormalities, and despite the numerous therapeutic advances remains an incurable pathology. Here we show that the RNA Polymerase II binding protein, Che-1/AATF is required for MM cells growth by sustaining genome wide transcription and the recruitment of Pol II to the DNA. Notably, we found that Che-1 localizes on active chromatin and its depletion leads to accumulation of heterochromatin by a global decrease of histone acetylation. Moreover, we show that Che-1 directly interacts with histones and competes with HDAC class I members for histones binding. Strikingly, Che-1 downregulation decreases BRD4 chromatin accumulation and further sensitize MM cells to bromodomain and extra-terminal (BET) inhibitors. Overall, our findings identify an essential role of Che-1 in maintaining open chromatin required for sustaining MM growth, and support Che-1 as a possible target for MM therapy, alone or in combination with BET inhibitors.

2021-06-07 | GSE100646 | GEO

Che-1/AATF sustains cell growth and global transcription in Multiple Myeloma through the maintenance of open chromatin structure [ATAC-Seq]

2021-06-07 | GSE100494 | GEO

Che-1/AATF sustains cell growth and global transcription in Multiple Myeloma through the maintenance of open chromatin structure [RNA-Seq]

2021-06-07 | GSE100493 | GEO

Che-1/AATF sustains cell growth and global transcription in Multiple Myeloma through the maintenance of open chromatin structure. [RNA-Seq, mouse]

Project description:This series contains the transcriptomic profiling for control and Che-1 silencing in the Multiple Myeloma mice model’s.

2021-06-07 | GSE120517 | GEO

Che-1/AATF sustains cell growth and global transcription in Multiple Myeloma through the maintenance of open chromatin structure. [ATAC-Seq, mouse]

Project description:This series contains the ATAC-seq data for control and Che-1 silencing in the Multiple Myeloma mice model.

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AATF interactome in human Kms27 cells

Project description:Multiple myeloma is a hematological neoplasm of plasma cells characterized by an abnormal production of immunoglobulins. Che-1/AATF (Che-1) is an RNA binding protein involved in transcription regulation and highly expressed in this malignancy. In order to better characterize its functions in this type of disease, we performed co-immunoprecipitation experiments coupled with a mass-spec analysis by using total MM Kms27 cell lysates. From this analysis we were able to identify more than 600 proteins significantly enriched in Che-1 precipitates. As expected from its role in ribosomal RNA maturation, the Che-1 interactome contains numerous proteins involved in pre-rRNA processing and several ribosomal proteins. Notably, among other proteins interacting with Che-1, we identified many essential components of the paraspeckle which play an important role in the regulation of gene expression.

2022-08-03 | PXD026659 | Pride

Che-ating death: CHE1/AATF protects from p53-mediated apoptosis.

Project description:The tumour suppressor p53 directs cells towards different fates depending on the cell type and the stimulus. The decision to direct a cell towards apoptosis rather than cell-cycle arrest or senescence has important implications for tumour suppression in normal cells and drug response in tumour cells. Cells that undergo senescence and growth arrest can persist and contribute to organismal ageing (Campisi, 2005), or they can contribute to tumour relapse (Jackson et al, 2012). In this issue of The EMBO Journal, Höpker et al (2012) show in a comprehensive study that the RNA PolII binding protein CHE1/AATF is a factor that determines the fate of cells that have activated the p53 pathway.

| S-EPMC3474930 | biostudies-other

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