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Association of Environmental Exposure to Chromium with Differential DNA Methylation: An Epigenome-Wide Study

ABSTRACT: To identified the differential methylation of genes and regions to elucidate the mechanisms of toxicity related to environmental chromium exposure. Fasting whole blood samples were collected by clinicians in ethylenediaminetetraacetic acid (EDTA) anticoagulant tubes, and stored them at −80 °C. A DNeasy Blood and Tissue Kit (Qiagen) was used to isolate DNA from whole blood. The purity and concentration of DNA were estimated using Nanodrop 2000 (ThermoScietific). Approximately 500 ng of genomic DNA from each sample was used for sodium bisulfite conversion with the EZ DNA methylation Gold Kit (Zymo Research, USA) in accordance with the manufacturer’s standard protocol. Genome-wide DNA methylation was performed using the Illumina Infinium HumanMethylation850K (EPIC) BeadChip (Illumina Inc, USA) in accordance with the manufacturer’s instructions.

ORGANISM(S): Homo sapiens

PROVIDER: GSE206418 | GEO | 2022/12/13

REPOSITORIES: GEO

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