RNA pull-down analysis of hsa_circ_0001400 in 293T cells
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ABSTRACT: 293T cells are transfected with hsa_circ_0001400 expression plasmid vectors for 24 hours. Cells are then incuvated with Psoralen, exposed to long-wave UV for 10 minutes, and total RNA was extracted. Biotynilated DNA oligos sense (control) or antisense of circ_0001400 are added and circRNA-RNA complexes are enriched using streptavidin beads. RNA was cleaned and exposed to short-wave UV and subjected for RNA-Seq.
ORGANISM(S): Homo sapiens
PROVIDER: GSE206929 | GEO | 2022/12/19
REPOSITORIES: GEO
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