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Real-time quantitative PCR analysis of human astrocytes exposed to pressure-driven flow and/or Direct Current Stimulation in vitro

ABSTRACT: The objective of this study was to investigate the effect of Direct Current Stimulation (DCS) on the gene expression of human astrocytes (HA). DCS at 0.1 or 1mA was applied to monolayers of HA for 10 minutes. Expression of a set of neuroactive genes was assessed immediately of 1 hour after DCS using RT-qPCR. Because DCS can produce electroosmotic flow and fluid shear stress known to influence cell function, we compared three interventions: pressure-driven flow across the monolayer alone (conP), pressure-driven flow plus DCS (DCS P), and DCS alone with flow blocked (DCS S).

ORGANISM(S): Homo sapiens

PROVIDER: GSE207140 | GEO | 2022/06/29

REPOSITORIES: GEO

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