Genomics

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Distribution of RNA polymerase II and changes of carbo-terminal phosphorylation sites after INTS11 knockdown


ABSTRACT: As Integrator is tightly associated with RNAPII-CTD, it is critical to understand how the RNAPII engaged and conducted within the active gene promoter for divergent transcription. We thus employed RNAPII ChIP-seq (Chromatin Immuno-precipitation with RNAPII antibody) to determine the distribution of the total RNAPII and its phosphorylation isoforms. Total RNA polymerase II and its carbon terminal phosphorylation(RNA polymerase II-ctd-Tyr-1,RNA polymerase II-ctd-ser-2) before and after INTS11 knockdown in HCT116-INTS11-AID cells changes chromatin immunoprecipitation DNA sequencing (ChIP-seq).

ORGANISM(S): Homo sapiens

PROVIDER: GSE207269 | GEO | 2023/07/25

REPOSITORIES: GEO

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