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ELL3 promotes stem cell native to primed transition through LINE-based enhancers

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Mus musculus

PROVIDER: GSE208227 | GEO | 2023/06/20

REPOSITORIES: GEO

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ELL3 promotes stem cell native to primed transition through LINE-based enhancers [4C-seq]

Project description:Long interspersed nuclear elements-1 (LINE-1) are the clade of retrotransposons still possessing autonomous mobility in mammals. LINE-1 transcription contributed to global chromatin opening in mouse early embryos. Whether LINE-1 element plays a direct role in the local regulation of its nearby genes remains largely obscure. ELL3 occupies and represses the youngest LINE-1 family members L1Md_T and L1Md_Gf in naïve mouse ES cells. We found that the enhancer function of the evolutionally young LINE-1s, and suggests that ELL3 act as a key drivers of the naïve-primed transition through regulating the young LINE-1 based enhancers.

2023-06-20 | GSE229447 | GEO

ELL3 promotes stem cell native to primed transition through LINE-based enhancers [ChIP-Seq]

Project description:Long interspersed nuclear elements-1 (LINE-1) are the clade of retrotransposons still possessing autonomous mobility in mammals. LINE-1 transcription contributed to global chromatin opening in mouse early embryos.Whether LINE-1 element plays a direct role in the local regulation of its nearby genes remains largely obscure.ELL3 occupies and represses the youngest LINE-1 family members L1Md_T and L1Md_Gf in naïve mouse ES cells.We found that the enhancer function of the evolutionally young LINE-1s, and suggests that ELL3 act as a key drivers of the naïve-primed transition through regulating the young LINE-1 based enhancers.

2023-06-20 | GSE207397 | GEO

ELL3 promotes stem cell native to primed transition through LINE-based enhancers [RNA-Seq]

Project description:Long interspersed nuclear elements-1 (LINE-1) are the clade of retrotransposons still possessing autonomous mobility in mammals. LINE-1 transcription contributed to global chromatin opening in mouse early embryos.Whether LINE-1 element plays a direct role in the local regulation of its nearby genes remains largely obscure.ELL3 occupies and represses the youngest LINE-1 family members L1Md_T and L1Md_Gf in naïve mouse ES cells.We found that the enhancer function of the evolutionally young LINE-1s, and suggests that ELL3 act as a key drivers of the naïve-primed transition through regulating the young LINE-1 based enhancers.

2023-06-20 | GSE208226 | GEO

ELL3 promotes stem cell native to primed transition through LINE-based enhancers [ATAC-seq]

Project description:Long interspersed nuclear elements-1 (LINE-1) are the clade of retrotransposons still possessing autonomous mobility in mammals. LINE-1 transcription contributed to global chromatin opening in mouse early embryos.Whether LINE-1 element plays a direct role in the local regulation of its nearby genes remains largely obscure.ELL3 occupies and represses the youngest LINE-1 family members L1Md_T and L1Md_Gf in naÃ¯ve mouse ES cells.We found that the enhancer function of the evolutionally young LINE-1s, and suggests that ELL3 act as a key drivers of the naÃ¯ve-primed transition through regulating the young LINE-1 based enhancers.

2023-06-20 | GSE225176 | GEO

ELL3 promotes stem cell native to primed transition through LINE-based enhancers

Project description:ELL3 promotes stem cell native to primed transition through LINE-based enhancers

| PRJNA858808 | ENA

ELL3 promotes stem cell native to primed transition through LINE-based enhancers [ATAC-seq]

Project description:ELL3 promotes stem cell native to primed transition through LINE-based enhancers [ATAC-seq]

| PRJNA934293 | ENA

ELL3 promotes stem cell native to primed transition through LINE-based enhancers [4C-seq]

Project description:ELL3 promotes stem cell native to primed transition through LINE-based enhancers [4C-seq]

| PRJNA954447 | ENA

ELL3 promotes stem cell native to primed transition through LINE-based enhancers [RNA-Seq]

Project description:ELL3 promotes stem cell native to primed transition through LINE-based enhancers [RNA-Seq]

| PRJNA858811 | ENA

ELL3 promotes stem cell native to primed transition through LINE-based enhancers [ChIP-Seq]

Project description:ELL3 promotes stem cell native to primed transition through LINE-based enhancers [ChIP-Seq]

| PRJNA855236 | ENA

Ell3 enhances differentiation of mouse embryonic stem cells by regulating epithelial-mesenchymal transition and apoptosis.

Project description:Ell3 is a testis-specific RNA polymerase II elongation factor whose cellular function is not clear. The present study shows that Ell3 is activated during the differentiation of mouse embryonic stem cells (mESCs). Furthermore, Ell3 plays a critical role in stimulating lineage differentiation of mESCs by promoting epithelial-mesenchymal transition (EMT) and suppressing apoptosis. Mouse ESCs engineered to stably express Ell3 were rapidly differentiated compared with control cells either under spontaneous differentiation or neural lineage-specific differentiation conditions. Gene expression profile and quantitative RT-PCR analysis showed that the expression of EMT markers, such as Zeb1 and Zeb2, two major genes that regulate EMT, was upregulated in Ell3-overexpressing mESCs. Remarkably, knockdown of Zeb1 attenuated the enhanced differentiation capacity of Ell3-overexpressing mESCs, which indicates that Ell3 plays a role in the induction of mESC differentiation by inducing EMT. In contrast to Ell3-overexpressing mESCs, Ell3-knock down mESCs could not differentiate under differentiation conditions and, instead, underwent caspase-dependent apoptosis. In addition, apoptosis of differentiating Ell3-knock out mESCs was associated with enhanced expression of p53. The present results suggest that Ell3 promotes the differentiation of mESCs by activating the expression of EMT-related genes and by suppressing p53 expression.

| S-EPMC3386972 | biostudies-literature

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