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HypoMap – a unified single cell gene expression atlas of the murine hypothalamus

ABSTRACT: The hypothalamus plays a key role in coordinating a plethora of fundamental body functions, such as energy homeostasis, social behavior and sleep. Despite recent progress in single-cell technologies, we still lack a unified catalogue and molecular characterization of the heterogeneous cell types and, specifically, neuronal subtypes in this brain region. Here, we combined 17 publicly available hypothalamic single cell sequencing datasets and in-house single-nuclear sequencing of 36,626 cells to create 'HypoMap', an integrated reference atlas of the murine hypothalamus of 384,925 cells. We have leveraged the power of this vast amount of available data by combining them in a single platform, which allows the future incorporation of additional experiments. We validated HypoMap by comparing data collected from SmartSeq2 and bulk RNA sequencing of selected neuronal cell types with different degrees of cellular heterogeneity. Finally, we identified novel classes of glucagon-like peptide-1 receptor (Glp1r)- and Prepronociceptin (Pnoc-) expressing neurons and validated them using single-molecule in situ hybridization. Collectively, HypoMap provides a unified framework for the systematic functional annotation of murine hypothalamic cell types, and will serve as an important platform for future studies to further unravel the functional organization of hypothalamic neurocircuits, as well as identifying novel druggable targets for treating metabolic disorders.

ORGANISM(S): Mus musculus

PROVIDER: GSE208355 | GEO | 2022/10/13

REPOSITORIES: GEO

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