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Epigenetically-controlled tumor antigens derived from splice junctions between exons and transposable elements [RNA-seq]

ABSTRACT: To study the expression of junctions between exons and transposable elements (TEs), we generated paired-end RNAseq from various mouse tumor cell lines, with several biological replicates (cultures from different days). To analyse regulation of JET expression, we generated Setdb1-deficient B16F10 and B16OVA cells, using lentivirus-based CRISPR/Cas9. Ctrl and KO cells lines were used to generate paired-end RNAseq, with several biological replicates (cultures on different days). We generated paired-end RNAseq from healthy female mouse tissues, to compare JET expression in healthy and tumor cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE208388 | GEO | 2022/11/22

REPOSITORIES: GEO

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