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Lineage tracing and single nuclear multiomics reveal novel features of adaptive and maladaptive repair after acute kidney injury

ABSTRACT: We combined lineage tracing of cycling (Ki67+) cells with single nuclear multiomics (single nucleus RNA-seq + single nucleus ATAC-seq) to characterize the long-term (4 weeks and 6 months) outcome of cells that initiate proliferation early after acute kidney injury (AKI). The data document a broad proliferative response to injury in epithelial and non-epithelial kidney cell types, identify novel transcription factors governing the adaptive and maladaptive proximal tubule cell state and highlight the importance of enhancer dynamics in determining cell states. Comparison of lineage traced with control proximal tubule cells reveals long-term effects of AKI on proximal tubule cells, even following adaptive repair.

ORGANISM(S): Mus musculus

PROVIDER: GSE209610 | GEO | 2022/11/22

REPOSITORIES: GEO

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