Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Single cell RNA sequencing of macrophage-depleted mammary gland by clodronate liposome treatment

ABSTRACT: To investigate the impact of macrophages on cell population of mammary gland, we performed the single cell RNA sequencing of the macrophage-depleted mammary gland compared with control.

ORGANISM(S): Mus musculus

PROVIDER: GSE210825 | GEO | 2023/04/20

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

single cell RNA sequencing of macrophage-depleted mammary gland by clodronate liposome treatment

Project description:single cell RNA sequencing of macrophage-depleted mammary gland by clodronate liposome treatment

| PRJNA867680 | ENA

Mammary gland

Project description:Goat Mammary gland RNA-Seq (DGE)

| PRJNA342566 | ENA

Resident macrophages promote homeostasis of hyaluronan-associated extracellular matrix in the mammary gland stroma

Project description:Tissue resident macrophages in the mammary gland are found in close association with epithelial structures and within the adipose stroma, and are important for mammary gland development and tissue homeostasis. While epithelial-associated macrophages have been linked to ductal development, the contributions of stromal macrophages to mammary gland homeostasis remain unknown. Using transcriptional profiling, we identify a distinct resident stromal macrophage subpopulation that is characterized by expression of Lyve-1, a receptor for the extracellular matrix component hyaluronan. This subpopulation is enriched in genes associated with extracellular matrix remodeling and is found to be specifically associated with hyaluronan-rich regions within the mammary stroma. Furthermore, macrophage depletion leads to increased accumulation of hyaluronan-associated extracellular matrix in the mammary stroma. These results demonstrate the presence of a distinct subpopulation of macrophages and provide insights into the functional contributions of these macrophages to stromal homeostasis in the mammary gland.

2020-05-31 | GSE148207 | GEO

Exosomal protein identification from untreated mice and macrophage-depleted mice

Project description:Exosomes are important mediators for cell-cell communication. In previous study, the clearance of exosomes were markedly delayed in systemic circulation of macrophage-depleted mice. To reveal the possible molecular regulation involved in exosome clearance, we performed exosomal proteomic analysis on MP-Exo from macrophage depleted mice and control ones.

2020-02-27 | PXD012885 | JPOST Repository

C/EBPβ deletion in macrophages impairs alveolar budding by altering the Wnt landscape during mammary gland development

Project description:Macrophages have important roles in mammary gland development and tissue homeostasis, but the specific mechanisms that regulate macrophage function need further elucidation. We have identified C/EBPβ as an important macrophage factor that is present in multiple populations of the normal mammary gland. Mammary glands from mice with C/EBPβ-deficient macrophages showed a significant decrease in alveolar budding during the estrus cycle. Deletion of C/EBPβ from macrophages resulted in changes in cytokine production, and an inability of both epithelial cells and macrophages to respond progesterone. RNA sequencing showed significant changes in PR-responsive genes and alterations in the Wnt landscape of epithelial cells in mammary glands that lacked C/EBPbeta in macrophages, which have been shown to mediate stem cell expansion during diestrus. These studies suggest that C/EBPbeta is a critical macrophage factor that facilitates macrophage-epithelial crosstalk during a key stage of mammary gland tissue homeostasis, and have critical implications for how disruption of tissue homeostasis can lead to tumorigenesis.

2024-07-16 | GSE225295 | GEO

Gene expression of myeloid lineage cells reveals a role for myeloid progenitor cells in mammary gland fibrosis in obesity

Project description:Obesity enhances macrophage recruitment in to mammary adipose tissue and increases fibrosis. We isolated monocytes/macrophages and myeloid progenitor cell populations from the mammary gland and queried gene expression of fibrosis related genes to assess the contribution of each population to fibrosis within the mammary gland.

2023-08-01 | GSE225841 | GEO

M/D-tumors vs non´-tumor mammary gland tissues

Project description:The main goal of this experiment was to contrast the gene expression of mammary gland tissues at three different tumoral stages : M/D-driven mammary gland small tumors vs mammary gland tissues that have been exposed to M/D but they did not develop a tumor (hyperplastic mammary gland) vs mammary gland tissues that were NOT expossed to M/D (normal mammary gland). Expression profile of 18 mice mammary gland tissues at 3 differents neoplastic stages before and after M/D expossure

2020-06-22 | GSE151197 | GEO

RNA-seq of goat mammary gland cells samples from different treatment groups

Project description:The goal of this project is to explore lncRNA55666 regulation goat mammary gland lipid metabolism. We tried to search the mechanism of lncRNA55666 regulation lipid metabolism. RNA-seq of goat mamamary gland cells samples from different groups: 5NC, lncRNA55666 overexpression, 3NC, lncRNA55666 knockdown. The goat mammary gland cells were cultured in 3D condition. The cell were transfected with virus with lncRNA55666 gene (overexpression), or inhibition of lncRNA expression (lncRNA gene knockdown).

2020-08-01 | GSE135692 | GEO

Mammary gland tumor immunoinfiltrate analysis

Project description:Mammary gland tumor immunoinfiltrate analysis

| PRJNA634093 | ENA

Mammary gland tumor immunoinfiltrate analysis

Project description:Mammary gland tumor immunoinfiltrate analysis

| PRJNA634255 | ENA