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Effect of depletion of ALKBH5 on gene expression of HaCAT human keratinocyte cell line

ABSTRACT: To investigate the function of ALKBH5 in the regulation of keratinocyte cellular function, we established HaCAT cell lines in which ALKBH5 has been knocked down by siRNA. We then performed gene expression profiling analysis using data obtained from RNA-seq of 4 different cells from duplicate experiments.

ORGANISM(S): Homo sapiens

PROVIDER: GSE211076 | GEO | 2022/08/12

REPOSITORIES: GEO

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Methylated RNA immunoprecipitation sequencing (MeRIP-seq) of human epidermis and HaCAT cells with or without ALKBH5 knockdown

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Effect of depletion of ALKBH5 on gene expression of HaCAT human keratinocyte cell line

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Protein dataset of immortalized HaCaT cells p53 knock down HaCaT keratinocytes

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Study of transcriptional effects of monosodium urate crystal (MSU) on HaCaT keratinocyte cell line.

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Project description:Clinical evidence has shown that electromagnetic fields produce a benefical therapeutic result for wounds, however little is still known about their exact mechanism of action. Moreover, clinical results concerning skin tissue restoration are still debated. In the present study, we carried out gene expression profiling of a human keratinocyte line (HaCaT) submitted to 1 hour of ELF-EMF (frequency 50Hz, intensity 1 mT) in order to identify up- and downregulated genes by this stimulus, and to verify the presence of specific molecular pathways activation. Most of the genes modulated were involved in mechanisms such as protein synthesis. In particular, these genes are related to Mammalian target of Rapamycin (mTOR), which has been identified as a kinase with a pivotal role in cellular proliferation and survival in mammals. In this study, we analyzed the expression profiles of 3 biological replicates of HaCaT cells exposed to ELF-EMF (frequency 50Hz, intensity 1 mT) for 1 hour. All HaCaT cells exposed to ELF-EMF RNAs were hybridized against control sham-exposed HaCaT cells RNAs. Each biological sample was repeated with a technical replicate (extraction-labeling) and a dye-swap experiment.

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