Transcriptomics

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RNA-seq analysis of differentially expressed genes between WT and shTRIM21 HCT116 cells.


ABSTRACT: To investigate the biological function TRIM21 in the colorectal cancer, we established HCT116 cell lines in which the TRIM21 gene has been knocked down by shRNA. We used a lentiviral shRNA technique to knockdown TRIM21 expression in HCT116 cells. A scrambled shRNA (shRNA/Control) was used as a control. We then performed RNA-Seq with Cloud-seq Biotech Inc. (Shanghai, China) to analyze gene expression changes in HCT116 cells. For each sample, three independent biological replicates were performed.

ORGANISM(S): Homo sapiens

PROVIDER: GSE211267 | GEO | 2023/05/23

REPOSITORIES: GEO

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