Transcriptomic analysis of pancreatic adenocarcinoma specimens obtained from Black and White patients

ABSTRACT: In pancreatic cancer clinical trials, Black patients are under-represented while having higher morbidity and mortality rates as compared to other racial groups. Multiple factors, including socioeconomic and lifestyle factors may contribute to this disparity, but genomic contributions remain unclear. In an exploratory project to identify genes that may contribute to differences in survival between Black and White patients with pancreatic cancer, transcriptomic sequencing of over 24,900 genes was performed in human pancreatic tumor and non-tumor tissue obtained from Black and White patients. Over 4,400 genes were differentially expressed in tumor and non-tumor tissue, irrespective of race. Of these 4,400 genes, four (AGR2, POSTN, TFF1, and CP) met the pre-defined statistical threshold for upregulation in pancreatic tumor tissue; these findings were confirmed by quantitative PCR. Transcriptomic analysis of pancreatic tumor tissue in Black and White patients revealed differential expression in 1,200 genes. Non-tumor and tumor gene expression differences within each race were assessed, revealing over 1,500 tumor-specific differentially expressed genes in pancreatic tumor and non-tumor tissue from Black patients. We identified TSPAN8 as a potential tumor-specific gene significantly overexpressed in pancreatic tumor tissue in Black patients as compared to White patients. Using Ingenuity Pathway Analysis software to compare the race-associated gene expression profiles, over 40 canonical pathways were identified to be potentially impacted by the gene expression differences between the races. Heightened expression of TSPAN8 was associated with poor overall survival, suggesting TSPAN8 as one potential genetic factor contributing to the differential outcomes in Black patients with pancreatic cancer, supporting the potential utility of larger genomic studies to further explore the role of TSPAN8 in pancreatic cancer.

ORGANISM(S): Homo sapiens

PROVIDER: GSE211398 | GEO | 2023/01/19

REPOSITORIES: GEO