Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Translatome profiling of warm temperature (35°C -> 38.5°C) treated mouse embryonic fibroblast cells in the presense or absence of PI3K inhibitor, 17β-hydroxy wortmannin

ABSTRACT: To see a possible effect of PI3K inhibition on a physiological warm temperature shift (WTS, 35°C to 38.5°C)-induced ribosomal accumulation on the Per2 minimal uORF, we performed Ribo-seq using dexamethasone-synchronized WTS-treated mouse embryonic fibroblast cells with or without 17β-hydroxy wortmannin (wortm) treatment at a circadain rising phase of Per2.

ORGANISM(S): Mus musculus

PROVIDER: GSE211532 | GEO | 2023/03/06

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Transcriptome and translatome profiling of warm temperature (35°C -> 38.5°C) treated mouse embryonic fibroblast cells

Project description:To see a possible effect of a physiological warm temperature shift (WTS, 35°C to 38.5°C) on Per2 transcription and translation in mammalian cells, we performed RNA-seq and Ribo-seq using dexamethasone-synchronized mouse embryonic fibroblast cells with or without WTS treatment at a circadain rising phase of Per2.

2023-03-06 | GSE188529 | GEO

Translatome profiling of warm temperature (35°C -> 38.5°C) treated mouse embryonic fibroblast cells in the presense or absence of PI3K inhibitor, 17β-hydroxy wortmannin

Project description:Translatome profiling of warm temperature (35°C -> 38.5°C) treated mouse embryonic fibroblast cells in the presense or absence of PI3K inhibitor, 17β-hydroxy wortmannin

| PRJNA870739 | ENA

Transcriptome and translatome profiling of warm temperature (35°C -> 38.5°C) treated mouse embryonic fibroblast cells

Project description:Transcriptome and translatome profiling of warm temperature (35°C -> 38.5°C) treated mouse embryonic fibroblast cells

| PRJNA779283 | ENA

Circadian ribosome profiling reveals a role for the Period2 upstream opening reading frame in sleep

Project description:Many mammalian proteins have circadian cycles of production and degradation, but de novo transcription is only responsible for a small fraction of this rhythmicity. We used ribosome profiling to quantify RNA translation in liver from circadian-entrained mice transferred to constant darkness conditions over a 24-h period and compared translation levels to absolute protein production for 16 circadian proteins. We observed a delay between translation and peak protein levels for several circadian genes covering a wide range of translation efficiencies. We found extensive binding of ribosomes to upstream open reading frames (uORFs) in circadian mRNAs, including the core clock gene Period2 (Per2). Increased uORFs in 5' UTRs was associated with decreased ribosome binding in downstream ORFs and reduced expression of synthetic reporter constructs in vitro and in single cells. Mutation of the Per2 uORF increased luciferase and fluorescence reporter expression in 3T3 cells without altering phase or period. Genomic Per2 uORF mutation using CRISPR/Cas9 increased PER2 expression in PER2:Luc MEFs and reduced sleep in Per2 uORF mutant mice. These results suggest that post-transcriptional processes shape translation of mRNA transcripts, which can impact physiological rhythms and sleep.

2023-05-05 | GSE201732 | GEO

Circadian ribosome profiling reveals a role for the Period2 upstream opening reading frame in sleep.

Project description:Many mammalian proteins have circadian cycles of production and degradation, and many of these rhythms are altered post-transcriptionally. We used ribosome profiling to examine post-transcriptional control of circadian rhythms by quantifying RNA translation in the liver over a 24-h period from circadian-entrained mice transferred to constant darkness conditions and by comparing ribosome binding levels to protein levels for 16 circadian proteins. We observed large differences in ribosome binding levels compared to protein levels, and we observed delays between peak ribosome binding and peak protein abundance. We found extensive binding of ribosomes to upstream open reading frames (uORFs) in circadian mRNAs, including the core clock gene Period2 (Per2). An increase in the number of uORFs in the 5'UTR was associated with a decrease in ribosome binding in the main coding sequence and a reduction in expression of synthetic reporter constructs. Mutation of the Per2 uORF increased luciferase and fluorescence reporter expression in 3T3 cells without altering the phase or period and increased luciferase expression in PER2:LUC MEF cells. Mutation of the Per2 uORF in mice increased PER2 protein levels and reduced total sleep time compared to that in wild-type mice. These results suggest that post-transcriptional processes shape translation of mRNA transcripts, which can impact physiological rhythms and sleep.

2023-09-13 | GSE231820 | GEO

Identification of warm-sensitive neuron markers using PhosphoTRAP

Project description:RNA-Seq profiling identifies transcripts enriched in preoptic neurons activated by warm temperature challenge

2016-09-12 | GSE80121 | GEO

3β-Hydroxy-friedelan-17β-carboxylic acid.

Project description:The title compound, C(30)H(50)O(3), which was isolated from a marine endophytic fungus, is a new friedelan derivative. The mol-ecule contains five six-membered rings, which exhibit boat (ring A), distorted boat (ring B) and chair (rings C-E) conformations. The crystal structure is stabilized by inter-molecular O-H⋯O hydrogen bonds, which link neighbouring mol-ecules into 12-membered rings.

| S-EPMC2961169 | biostudies-literature

MiRNA expression profile in human CD34+/CD105- cells derived from the renal artery subsequent to long warm ischemia compared short warm ischemia

Project description:MiRNA regulate the maintenance, differentiation and function of stem cells and progenitor cells. miRNA expression of progenitor cells located in the adventital layer of arterial vessels has not been characterized in either animal or human models. Further it is unknown if local arterial miRNA expression profiles change after injury of end organs supplied blood by these arterial conduits. CD34+/CD105- cells were extracted and analyzed for changes in miRNA expression after kidney specific ischemic injury. CD34+/CD105- cells were isolated from renal artery after short warm ischemic time in living donor kidney explants and long warm ischemic time following radical nephrectomy for renal cell cancer

2015-04-29 | E-GEOD-68366 | biostudies-arrayexpress

A conserved signaling complex controls warm temperature-mediated plant growth

Project description:The model plant Arabidopsis thaliana responds to mild high temperature by increased elongation growth of organs to enhance cooling capacity, in a process called thermomorphogenesis. Our understanding of the genetic regulation of thermomorphogenesis has increased in recent years. However, hardly anything is known about molecular mechanisms outside A. thaliana and cellular signaling pathways have been underexplored. Therefore, we mapped changes in protein phosphorylation in A. thaliana and crops exposed to warm temperature. Based on these results, we identified and characterized a novel, functionally conserved signaling complex of MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE KINASEs (MAP4KS) in warm temperature-mediated growth regulation in plants. This contributes to our understanding of warm temperature signaling, and can help guarantee food security under a changing climate.

2021-03-25 | PXD015468 | Pride

A conserved signaling complex controls warm temperature-mediated plant growth

2021-03-25 | PXD015483 | Pride