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Changes in gene expression of human monocyte derived dendritic cells exposed to live Borrelia burdgorferi or LTA

ABSTRACT: Dendritic cells bridge the innate and adaptive immune response by serving as sensors of infection and as the primary antigen presenting cells responsible for the initiation of the T cell response against invading pathogens. Initial interactions between B. burgdorferi, the causative agent of Human Lyme disease, and dendritic cells remain largely unexplored. To address this, we cultured monocyte-derived dendritic cells (moDCs) with and without the Toll-Like Receptor 2 agonist LTA or live B. burgdorferi and examined changes in gene expression using RNAseq. We observed that B. burgdorferi exposed moDCs display a distinct and novel gene expression signature that differs from that induced by the Toll-Like Receptor 2 reference agonist. Collectively these studies indicate that the interaction of live B. burgdorferi with mo-DCs promotes a unique mature DC phenotype that likely impacts the nature of the adaptive T cell response generated in human Lyme disease.

ORGANISM(S): Homo sapiens

PROVIDER: GSE211551 | GEO | 2023/06/02

REPOSITORIES: GEO

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