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Expression profile of TTHA1438-deficient Thermus thermophilus HB8 strain

ABSTRACT: In order to find function of TTHA1438 protein from T. thermophilus HB8, we observed the expression profile of the total mRNA of the TTHA1438-deficient strain grown in a rich medium at 70°C for 240, 360, 480, 600 and 760 min.

ORGANISM(S): Thermus thermophilus HB8

PROVIDER: GSE21198 | GEO | 2010/10/15

SECONDARY ACCESSION(S): PRJNA129459

REPOSITORIES: GEO

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Expression profile of TTHA1438-deficient Thermus thermophilus HB8 strain

Project description:In order to find function of TTHA1438 protein from T. thermophilus HB8, we observed the expression profile of the total mRNA of the TTHA1438-deficient strain grown in a rich medium at 70Â°C for 240, 360, 480, 600 and 760 min. Two TTHA1438-deficient T. thermophilus HB8 strains were each pre-cultured at 70Â°C for 16 h in 3 ml of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 ml) were inoculated into 1 liter of the same medium and then cultivated at 70Â°C. Cells were collected after 240, 360, 480, 600, 680 and 760 min, respectively, and then, the expression of each mRNA was analyzed.

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Time course of the mRNA expression in wild-type Thermus thermophilus HB8 strain grown on a rich medium.

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Time course of the mRNA expression in wild-type Thermus thermophilus HB8 strain grown in a rich medium (labeling:Affymetrix)

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Expression profile of TTHA0175-deficient Thermus thermophilus HB8 strain

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