Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression profile magnetic beads sorted CD8+ T cells from naïve splenocytes and OVA257-264 peptide activated splenocytes from WT and Susd2-/- OT-I mice

ABSTRACT: CD8+ T cells are phenotypically heterogeneous with varying degrees of functional change. We used Bulk RNA sequencing ( Bulk RNA-seq) to analyze the differences betwteen WT and Susd2-/- OT-I mice.

ORGANISM(S): Mus musculus

PROVIDER: GSE212179 | GEO | 2022/08/28

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Gene expression profile magnetic beads sorted CD8+ T cells from naïve splenocytes and OVA257-264 peptide activated splenocytes from WT and Susd2-/- OT-I mice

Project description:Gene expression profile magnetic beads sorted CD8+ T cells from naïve splenocytes and OVA257-264 peptide activated splenocytes from WT and Susd2-/- OT-I mice

| PRJNA874422 | ENA

Transcriptional effects of IL-6 on peptide-stimulated OT-I CD8 T cells after 2 or 7 days

Project description:Interleukin 6 (IL-6) is a pleiotropic cytokine with diverse roles in homeostasis, inflammation, and cancer. To identify transcriptional patterns and differentiation states induced by IL-6 in CD8 T cells, we performed RNAseq profiling of murine OT-I CD8+ T cells stimulated with SIINFEKL peptide in the presence of exogenous IL-6 (10 ng/ml) or anti-IL6R antibody (to block endogenous IL-6 signaling). Bulk OT-I splenocytes were stimulated with SIINFEKL peptide and CD8 T cells were FACS-sorted for RNA extraction after 2 and 7 days. We found that IL-6 potently repressed classical effector differentiation and promoted a gene expression pattern similar to that of memory precursor cells.

2022-11-04 | GSE199044 | GEO

Gene expression profiles of peptide-primed OT-1 cells with or without corticosteroid

Project description:We report the gene expression profiles of FACS-sorted OT-I cells stimulated with N4 or Y3 peptide with or without corticosteroids.

2019-08-24 | GSE136236 | GEO

Desulfosporosinus sp. OT strain:OT

Project description:Desulfosporosinus sp. OT genome sequencing

| PRJNA61253 | ENA

Pseudomonas alcaligenes OT 69 strain:OT 69

Project description:Pseudomonas alcaligenes OT 69 strain:OT 69 Genome sequencing and assembly

| PRJNA202928 | ENA

RNAseq of control OT-I cells and Eomes-overexpressing OT-I cells

Project description:High amount of Eomes might drive T cell exhaustion. In order to understand how Eomes contributes to exhaustion of CD8+ T cell in the TME, we conducted transcriptional analysis of control OT-I cells and Eomes-overexpressing OT-I cells

2018-11-26 | GSE122889 | GEO

Transcription profiling of mouse splenocytes from OT-1 TCR transgenic mice to assess the effect of IL-12 on CTL gene expression

Project description:The goal was to determine how IL-12 affects gene expression by murine CTL. Experiment Overall Design: Following RBC lysis, splenocytes from OT-1 TCR transgenic mice (5 million/ml) were cultured in six well-plates (5-6ml/well) in IMDM supplemented with 10% FCS and 55Î¼M 2-ME with 1Î¼M SIINFEKL. After 4 days, the cells were harvested by purification with Ficoll-Paque PLUS and placed in fresh medium containing 0 or 20ng/ml rmIL-12. Twenty-four hours later the cells were harvested and used in experiments. The live cells harvested were >98% CD8+VÎ±2+ (OT-1).

2009-02-08 | E-GEOD-13173 | biostudies-arrayexpress

anti-Eomes ChIPseq of control OT-I cells and Eomes-overexpressing OT-I cells

Project description:High amount of Eomes might drive T cell exhaustion. In order to understand how Eomes contributes to exhaustion of CD8+ T cell in the TME as a transcription factor, we conducted anti-Eomes ChIPseq analysis of control OT-I cells and Eomes-overexpressing OT-I cells.

2018-11-26 | GSE122888 | GEO

Memory OT-I cells

Project description:Memory OT-I cells

| PRJNA639155 | ENA

OT-I T cell activation in Lrrc8af/f and Lrrc8af/f Cd4-Cre;OT-I mice

Project description:Splenocytes were separated from Lrrc8af/f and Lrrc8af/f Cd4-Cre;OT-I mice. The splenocytes were stimulated with indicated peptides (unstime/ SIINFEKL-10pM(NL)/ SIINFEKL-1000pM(NH)/ SIIGFEKL-1000nM(G4)) for 6 hours. The OT-I CD8+ T cells were labeled with Va2-PE/CD8-A700 and sorted for the sequencing.

2023-09-26 | GSE243985 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data