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The N-terminal extension of Arabidopsis ARGONAUTE 1 is essential for microRNA activities

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE212365 | GEO | 2023/02/21

REPOSITORIES: GEO

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The N-terminal extension of Arabidopsis ARGONAUTE 1 is essential for microRNA activities II

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-02-21 | GSE224481 | GEO

The N-terminal extension of Arabidopsis ARGONAUTE 1 is essential for microRNA activities.

Project description:microRNAs (miRNAs) regulate target gene expression through their ARGONAUTE (AGO) effector protein, mainly AGO1 in Arabidopsis thaliana. In addition to the highly conserved N, PAZ, MID and PIWI domains with known roles in RNA silencing, AGO1 contains a long, unstructured N-terminal extension (NTE) of little-known function. Here, we show that the NTE is indispensable for the functions of Arabidopsis AGO1, as a lack of the NTE leads to seedling lethality. Within the NTE, the region containing amino acids (a.a.) 91 to 189 is essential for rescuing an ago1 null mutant. Through global analyses of small RNAs, AGO1-associated small RNAs, and miRNA target gene expression, we show that the region containing a.a. 91-189 is required for the loading of miRNAs into AGO1. Moreover, we show that reduced nuclear partitioning of AGO1 did not affect its profiles of miRNA and ta-siRNA association. Furthermore, we show that the 1-to-90a.a. and 91-to-189a.a. regions of the NTE redundantly promote the activities of AGO1 in the biogenesis of trans-acting siRNAs. Together, we report novel roles of the NTE of Arabidopsis AGO1.

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The N-terminal extension of Arabidopsis ARGONAUTE 1 is essential for microRNA activities [mRNA]

Project description:We profiled gene expression of AGO1 NTE truncation mutant lines to study the roles of the NTE region in Arabidopsis AGO1 involved RNA-induced silencing complex (RISC) formation.

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The N-terminal extension of Arabidopsis ARGONAUTE 1 is essential for microRNA activities [ncRNA_seq]

Project description:We profiled the small RNA of AGO1 NTE truncation mutant lines, and the small RNA associated with AGO1 NTE truncation mutant to study the roles of the NTE region in Arabidopsis AGO1 involved RNA-induced silencing complex (RISC) formation.

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The N-terminal extension of Arabidopsis ARGONAUTE 1 is essential for microRNA activities [RNA_seq]

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The N-terminal extension of Arabidopsis ARGONAUTE 1 is essential for microRNA activities

Project description:The N-terminal extension of Arabidopsis ARGONAUTE 1 is essential for microRNA activities

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The N-terminal extension of Arabidopsis ARGONAUTE 1 is essential for microRNA activities [mRNA]

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