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Genome-wide identification and expression profiles of CsHIPP family genes under heavy metal stress in Camellia sinensis (L.)

ABSTRACT: HIPP (heavy metal associated isoprenylated plant protein) is a group of metal-binding metallochaperones, which plays crucial roles in metal homeostasis and detoxification. Up to now, little information of CsHIPP family genes in C. sinensis has been reported. In present study, a total of 56 CsHIPP genes from C. sinensis were identified, which were unevenly distributed on 15 chromosomes. The CsHIPP family genes were divided into five groups based on the topology of phylogenetic tree and identified AtHIPPs and OsHIPPs. Motif and gene structure analysis showed that the members in the same group showed similar conserved motifs and intron/exon structure. The expression pattern of CsHIPP family genes in different organs of C. sinensis was investigated using the previous RNA-seq data. The expression level of CsHIPP genes in C. sinensis under Cd and As treatments was also explored based on our RNA-seq analysis. The results showed that 16 CsHIPP genes displayed the same expression pattern under Cd and As treatments, which were also verified by qRT-PCR. Among them, three CsHIPP genes (CsHIPP15, CsHIPP36, and CsHIPP4) were increased at both 10 d and 15 d after Cd and As treatments, and one gene (CsHIPP40) was increased to a maximum at 10 d, and decreased at 15 d of Cd and As treatments, indicating that these four CsHIPP genes in C. sinensis might be involved in the resistance to Cd and As stresses. These results provide a basis for the functional characterization of CsHIPP genes and investigations on the molecular mechanisms of heavy metal homeostasis in C. sinensis.

ORGANISM(S): Camellia sinensis

PROVIDER: GSE212827 | GEO | 2022/09/09

REPOSITORIES: GEO

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