Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression analysis of S. Typhimurium cells residing in the food vacuole (phagosome) of the bacteria grazing ciliate Tetrahymena

ABSTRACT: The gene expression of Salmonella enterica Typhimurium MB282 residing in the food vacuole (phagosome) of Tetrahymena was analyzed by microarray.

ORGANISM(S): Salmonella enterica

PROVIDER: GSE21308 | GEO | 2010/04/13

SECONDARY ACCESSION(S): PRJNA126559

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Gene expression analysis of S. Typhimurium cells residing in the food vacuole (phagosome) of the bacteria grazing ciliate Tetrahymena

Project description:The gene expression of Salmonella enterica Typhimurium MB282 residing in the food vacuole (phagosome) of Tetrahymena was analyzed by microarray. A minimum of three biological replicates were used. Each biological rep was comprised of at least two technical replicate arrays. MB282 incubated in H2O and MB282 grown to mid - log in LB were used as controls.

2010-04-13 | E-GEOD-21308 | biostudies-arrayexpress

Transcriptome analysis of the binucleate ciliate Tetrahymena thermophila with asynchronous nuclear cell cycles

Project description:As a prototypic ciliated protozoan, Tetrahymena thermophila harbors two functionally and physically distinct nuclei within a shared cytoplasm. During the vegetative phase of the life cycle, the S phases of the diploid germline micronucleus and polyploid somatic macronucleus are temporally offset. Here we report the first RNA-Seq analysis across the cell cycle of a binucleated ciliated protozoan. We then performed gene expression profiling analysis using data obtained from RNA-seq of 4 different cells at two time points.

2023-01-11 | GSE209619 | GEO

Tetrahymena thermophila

Project description:Tetrahymena thermophila

| PRJNA11958 | ENA

mTOR regulates phagosome and entotic vacuole fission.

Project description:Macroendocytic vacuoles formed by phagocytosis, or the live-cell engulfment program entosis, undergo sequential steps of maturation, leading to the fusion of lysosomes that digest internalized cargo. After cargo digestion, nutrients must be exported to the cytosol, and vacuole membranes must be processed by mechanisms that remain poorly defined. Here we find that phagosomes and entotic vacuoles undergo a late maturation step characterized by fission, which redistributes vacuolar contents into lysosomal networks. Vacuole fission is regulated by the serine/threonine protein kinase mammalian target of rapamycin complex 1 (mTORC1), which localizes to vacuole membranes surrounding engulfed cells. Degrading engulfed cells supply engulfing cells with amino acids that are used in translation, and rescue cell survival and mTORC1 activity in starved macrophages and tumor cells. These data identify a late stage of phagocytosis and entosis that involves processing of large vacuoles by mTOR-regulated membrane fission.

| S-EPMC3842999 | biostudies-literature

Tetrahymena

Project description:Nucleotide sequences of Tetrahymena species

| PRJEB23142 | ENA

Tetrahymena borealis

Project description:Tetrahymena borealis Transcriptome Sequencing

| PRJNA167916 | ENA

Tetrahymena elliotti

Project description:Tetrahymena elliotti Transcriptome Sequencing

| PRJNA167917 | ENA

Tetrahymena malaccensis

Project description:Tetrahymena malaccensis Transcriptome Sequencing

| PRJNA167918 | ENA

Characterization of the CORVET complexes of Tetrahymena thermophila

Project description:In endolysosomal networks, two hetero-hexameric tethers called HOPS and CORVET are found widely throughout eukaryotes. The ciliate Tetrahymena thermophila is a unicellular organism with elaborate endolysosomal pathways. Curiously, Tetrahymena and related protozoa have lost HOPS. Tetrahymena encodes multiple paralogs of most CORVET subunits, assembled into six distinct hexameric complexes, including a distinct Vps8 subunit. Here we analyze the composition of these complexes immunoisolated by affinity capture using the Vps8-FLAG paralogs as bait.

2019-12-19 | PXD014895 | Pride

Salmonella enterica

Project description:Gene expression analysis of S. Typhimurium cells residing in the food vacuole (phagosome) of the bacteria grazing ciliate Tetrahymena

| PRJNA126559 | ENA

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data