Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Vdelta2+ TCR sequencing of Vgamma9Vdelta2 T cells from adults and newborns

ABSTRACT: Vdelta2+ TCR sequencing of Vgamma9Vdelta2 T cells from adults and newborns

ORGANISM(S): Homo sapiens

PROVIDER: GSE213280 | GEO | 2022/09/16

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Newborn and child-like molecular signatures in older adults stem from TCR shifts across human lifespan

Project description:CD8+ T-cells provide robust anti-viral immunity, yet how epitope-specific T-cells evolve across the human lifespan is unknown. We defined CD8+ T-cell immunity directed at the prominent influenza epitope, HLA-A*02:01-M158-66 (A2/M158) across four age groups (newborns, children, adults and elderly) ex vivo at phenotypic, single cell sequence (transcriptomic), clonal and functional levels. We identified a linear differentiation trajectory from newborns to children then adults, followed by divergence and a clonal reset in older adults. Gene profiles in older adults closely resembled those observed in newborns and children, despite being clonally-different. However, only child- and adult-derived A2/M158+CD8+ T-cells had the potential to differentiate into highly cytotoxic epitope-specific CD8+ T-cells, which was linked to highly functional public TCRab-signatures. Suboptimal TCRab-signatures detected in older adults led to poorer proliferation, polyfunctionality, avidity and recognition of peptide mutants, although displayed no signs of exhaustion. Our study suggests that priming T-cells at different stages of life might greatly affect CD8+ T-cell responses towards viral infections.

2023-08-16 | GSE237817 | GEO

Single-cell transcriptomic reveals heterogeneous feature of myeloid-derived suppressor cells in newborns

Project description:In order to gain deep insights into the molecular characteristics and heterogeneity of MDSCs in human newborns, low-density CD11b+HLA-DR-/low immature myeloid cells from the peripheral blood of term infants, preterm infants, and adults control were subjected to single-cell RNA sequencing (scRNA-seq).

2024-06-06 | GSE253963 | GEO

Gene expression profile of activated CD4 T cells from adults and newborns

Project description:Given their precipitous encounter with the environment, newborn infants might be expected to possess abundant immunoprotective mechanisms. Paradoxically, their T cells display grossly impaired Th1 anti-bacterial and anti-viral responses. This study identifies factors produced by neonatal CD4 T cells when compared with adult naive CD4 T cells and highlights CXCL8 as a pivotal effector molecule in neonatal T cells. Using Affimetrix microarray we compared gene expression in cord blood derived CD4 T cells with naive CD4 T cells from adults after polyclonal stimulation.

2014-09-21 | GSE52129 | GEO

Foxp3+RORgt+ TCR Sequencing

Project description:TCR repertoire sequencing of mucosal Foxp3+RORgt+ regulatory T cells

| PRJEB15258 | ENA

Gene expression profile of activated CD4 T cells from adults and newborns

2014-09-21 | E-GEOD-52129 | biostudies-arrayexpress

Single-cell TCR-sequencing of T cells in crescentic glomerulonephritis

Project description:Single-cell TCR sequencing of T cells from ANCA-associated glomerulonephritis patients and experimental crescentic glomerulonephritis mouse model.

2023-03-31 | GSE223107 | GEO

Single-cell RNA-sequencing and single-cell TCR-sequencing of tumour-infiltrating CD8+ T cells derived from mouse BRAFV600E melanoma [scRNA-Seq + TCR 10x]

Project description:Single-cell RNA-sequencing and single-cell TCR-sequencing was used to characterize PGE2-mediated changes in the gene expression profile of tumor-infiltrating CD8+ T cells.

2024-02-21 | GSE231302 | GEO

TCR repertoire analysis by Next-generation sequencing of mouse CD8+ T cells in spleen or tumor

Project description:We have reported that intra-tumoral treatment with 1V270, a phospholipid-conjugated TLR7 agonist,induces local expansion an systemic dispersion of oligoclonal tumor-specific T cells by TCR repertoire analysis using next generation RNAseq methodology. Here, we examined whether systemic 1V270 therapy also induced oligoclonal expansion of tumor-specific T cells. Two groups of BALB/c mice (n=4/group) were i.p. treated with 1V270,a phospholipid-conjugated TLR7 agonist. One cohort of mice was i.v. injected with 4T1-GLF cells (2×104) on day 0. Another cohort did not receive i.v. tumor injection (no tumor-exposed mice). 4T1 cells were orthotopically inoculated on day 21. To examine clonal specificity of tumor-specific T cells, CD8+ cells were isolated from the spleens and the tumor infiltrating lymphocytes of secondarily challenged tumors after initial 1V270 therapy. The TCR repertoires were assessed by next generation RNA sequencing of both TCRαand TCR β genes.

2018-07-31 | E-MTAB-6566 | biostudies-arrayexpress

Alpha/Beta Mouse TCR Sequencing of CD4+ thymic regulatory and conventional T-cells

Project description:Thymic regulatory T cells (tTreg) are critical in maintenance of normal T cell immunity and tolerance. The role of TCR in tTreg cell selection remains incompletely understood. Here we assessed TCRa and TCRb sequences of tTreg and conventional thymic CD4+ T (Tconv) cells by high throughput sequencing. We identified ab TCR sequences that were unique to either tTreg or Tconv cells and found that these were distinct as recognized by machine learning (ML) algorithm and by preferentially used amino acid trimers in ab CDR3 of tTreg cells. In addition, a proportion of ab TCR sequences expressed by tTreg were also found in Tconv cells, and ML classified the great majority of these shared ab TCR sequences as characteristic of Tconv and not tTreg cells. These findings identify two populations of tTreg, one in which Treg fate is associated with unique properties of the TCR, and another with TCR properties characteristic of Tconv cells for which tTreg fate is determined by factors beyond TCR sequence.

2020-01-10 | GSE143291 | GEO

Transcriptome and TCR sequencing of T cells from metastectomies

Project description:Transcriptome and TCR sequencing of T cells from metastectomies

| PRJNA791117 | ENA