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Identification and removal of unexpected proliferative off-target cells emerging after implantation of iPSC-derived pancreatic islet cells

ABSTRACT: The differentiation of pancreatic endocrine cells from human pluripotent stem cells (PSC) has been thoroughly investigated for their application in cell therapy against diabetes. However, there is little information available on the long-term safety of these differentiated cells. Here we show that implantation of induced PSC-derived pancreatic islet cells (iPIC) results in the appearance of unexpected proliferative off-target cells via in vivo maturation. Since the unexpected cells have characteristics of both mesenchymal stem cells (MSC) and uterine tissue, we named these cells proliferative MSC and uterus like cells (PMUC). To detect PMUC without implantation, we developed four-week-extended culture system and found that PMUC can be reduced by compound treatment, especially a taxane Docetaxel. Indeed, when we implanted Docetaxel-treated iPIC, the appearance of PMUC disappeared. The present study provides useful insights into identifying and resolving safety issues which is particularly important in the field of cell-based medicine using PSC.

ORGANISM(S): Homo sapiens

PROVIDER: GSE213617 | GEO | 2024/04/22

REPOSITORIES: GEO

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