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BRPF1 co-occupies with H3K4me3 and H3K23ac in human ESCs essential to pluripotency

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Homo sapiens

PROVIDER: GSE213695 | GEO | 2022/12/21

REPOSITORIES: GEO

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BRPF1 co-occupies with H3K4me3 and H3K23ac in human ESCs essential to pluripotency [ATAC-seq]

Project description:Post-translational modifications (PTMs) on histones play essential roles in cell fate decisions during development. However, how these PTMs are recognized and coordinated remains to be fully illuminated. Here, we show that BRPF1, a multi-histone binding module protein, is essential to maintain pluripotency in human embryonic stem cells (ESCs). BRPF1, H3K4me3 and H3K23ac substantially co-occupy the active and stemness genes in hESCs. BRPF1 deletion impairs H3K23ac and leads to pluripotency exit and closed chromatin accessibility on stemness genes. Deletion of the N terminal or PHD-zinc knuckle-PHD (PZP) modules completely impairs BRPF1 to maintain hESC pluripotency while PWWP module deletion only partially impact its functions. Together, we reveal that the multi-histone binding module protein, BRPF1 co-ordinates the crosstalk between different histone modifications to maintain the pluripotency in hESCs.

2022-12-21 | GSE213690 | GEO

BRPF1 co-occupies with H3K4me3 and H3K23ac in human ESCs essential to pluripotency [ChIP-seq]

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BRPF1 co-occupies with H3K4me3 and H3K23ac in human ESCs essential to pluripotency [RNA-Seq]

2022-12-21 | GSE213694 | GEO

BRPF1 co-occupies with H3K4me3 and H3K23ac in human ESCs essential to pluripotency

Project description:BRPF1 co-occupies with H3K4me3 and H3K23ac in human ESCs essential to pluripotency

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BRPF1 co-occupies with H3K4me3 and H3K23ac in human ESCs essential to pluripotency [ATAC-seq]

Project description:BRPF1 co-occupies with H3K4me3 and H3K23ac in human ESCs essential to pluripotency [ATAC-seq]

| PRJNA882078 | ENA

BRPF1 co-occupies with H3K4me3 and H3K23ac in human ESCs essential to pluripotency [ChIP-seq]

Project description:BRPF1 co-occupies with H3K4me3 and H3K23ac in human ESCs essential to pluripotency [ChIP-seq]

| PRJNA882079 | ENA

BRPF1 co-occupies with H3K4me3 and H3K23ac in human ESCs essential to pluripotency [RNA-Seq]

Project description:BRPF1 co-occupies with H3K4me3 and H3K23ac in human ESCs essential to pluripotency [RNA-Seq]

| PRJNA882080 | ENA

SOX2 co-occupies distal enhancer elements with distinct POU factors in ESCs and NPCs to specify cell state.

Project description:SOX2 is a master regulator of both pluripotent embryonic stem cells (ESCs) and multipotent neural progenitor cells (NPCs); however, we currently lack a detailed understanding of how SOX2 controls these distinct stem cell populations. Here we show by genome-wide analysis that, while SOX2 bound to a distinct set of gene promoters in ESCs and NPCs, the majority of regions coincided with unique distal enhancer elements, important cis-acting regulators of tissue-specific gene expression programs. Notably, SOX2 bound the same consensus DNA motif in both cell types, suggesting that additional factors contribute to target specificity. We found that, similar to its association with OCT4 (Pou5f1) in ESCs, the related POU family member BRN2 (Pou3f2) co-occupied a large set of putative distal enhancers with SOX2 in NPCs. Forced expression of BRN2 in ESCs led to functional recruitment of SOX2 to a subset of NPC-specific targets and to precocious differentiation toward a neural-like state. Further analysis of the bound sequences revealed differences in the distances of SOX and POU peaks in the two cell types and identified motifs for additional transcription factors. Together, these data suggest that SOX2 controls a larger network of genes than previously anticipated through binding of distal enhancers and that transitions in POU partner factors may control tissue-specific transcriptional programs. Our findings have important implications for understanding lineage specification and somatic cell reprogramming, where SOX2, OCT4, and BRN2 have been shown to be key factors.

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SNF5 is an essential executor of epigenetic regulation in pluripotency and differentiation

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2013-01-01 | E-GEOD-36140 | biostudies-arrayexpress

Long range inter-chromosomal interaction of Oct4 distal enhancer loci regulates ESCs pluripotency

Project description:This SuperSeries is composed of the SubSeries listed below.

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