Transcriptomics

Dataset Information

0

Duxbl-mediated suppression of ZGA drives totipotency to pluripotency transition in mice III


ABSTRACT: In mammalian embryos, Dux transcription factors (TF) drive a cleavage stage-specific transcriptional burst associated with zygotic genome activation (ZGA) during which hundreds of genes and endogenous retroviral (ERV) elements are transiently expressed. In mice, ZGA begins in late 1-cell (1C) embryos and is shut down within hours at the late 2-cell (2C) to 4-cell (4C) stage. While this transition is accompanied by the loss of totipotency, it is not clear whether shutdown of Dux-induced ZGA is required for this process and how ZGA is terminated. Here, we reveal an essential negative feedback axis by which the Dux family member Duxbl terminates Dux-induced ZGA from the mid 2C stage onward by direct suppression of genomic Dux target loci. Consequently, Duxbl inactivation results in sustained expression of Dux-induced ZGA leading to 4C arrest. Our study reveals the Dux/Duxbl axis that determines the timing of totipotency exit enabling the very first divergence of cell fates.

ORGANISM(S): Mus musculus

PROVIDER: GSE213702 | GEO | 2023/12/07

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2023-12-07 | GSE213701 | GEO
2023-12-07 | GSE213700 | GEO
2023-12-07 | GSE234053 | GEO
2023-12-06 | GSE210891 | GEO
2023-12-06 | GSE210890 | GEO
2024-10-12 | GSE263217 | GEO
2023-06-01 | GSE222636 | GEO
2019-04-10 | GSE121746 | GEO
2019-02-23 | GSE126921 | GEO
2019-02-23 | GSE126920 | GEO