Genome-wide analysis of gene expression regulated by calcineurin-NFAT signaling in mouse embryonic stem cells
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ABSTRACT: Self-renewal and pluripotency are hallmarks of embryonic stem cells (ESCs). However, signaling pathways triggering their transition from self-renewal to differentiation are not well defined. Here, we report that the calcineurin-NFAT signaling pathway is both necessary and sufficient to switch ESCs from an undifferentiated state to early differentiation.To gain an insight into the function of calcinerin-NFAT signaling in ESC lineage commitment, we examined the repertoire of genes changed after LIF withdrawal alone, LIF withdrawal in the presence of cyclosporine A (CsA), a specific inhibitor of calcinerin and forced expression of active forms of calcineurin (deltaCnA) in the presence of LIF,and identified distinct classes of up and down regulated genes by calcineurin-NFAT signaling in mouse embryonic stem cells.
ORGANISM(S): Mus musculus
PROVIDER: GSE21378 | GEO | 2011/01/01
SECONDARY ACCESSION(S): PRJNA126093
REPOSITORIES: GEO
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