Transcriptomics

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Excess glucose or fat differentially affects gene expression and appetite regulation during zebrafish embryogenesis


ABSTRACT: Zebrafish embryos were exposed to 3% glucose (24-72 hpf) and injected 2 nL of BODIPY-FL C12:0 dissolved in canola oil directly into the yolk sac at 24 hpf. At 72 hpf embryos were collected for analysis. Quantitative PCR analysis of glucose exposed embryos demonstrated that glucose exposure activates the insulin pathway while FFA/TAG injection activates the lipolysis and beta-oxidation pathways. At 72 hpf embryos were collected and bulk RNAseq was performed on control, glucose exposed and FFA/TAGs injected embryos.

ORGANISM(S): Danio rerio

PROVIDER: GSE213859 | GEO | 2023/06/01

REPOSITORIES: GEO

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