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To investigate the mechanisms of CDCA regulates proliferation of Porcine Small Intestinal Epithelial Cells (IPEC-J2)

ABSTRACT: The objective of this study was to investigate the effects of CDCA on the gene expression of IPEC-J2 cells. The control group (CON) treated with PBS and the CDCA group treated with both CDCA. Compared with the CON group, CDCA group could regulate the gene expression related proliferation genes.

ORGANISM(S): Sus scrofa

PROVIDER: GSE214153 | GEO | 2022/12/09

REPOSITORIES: GEO

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Transcriptome-wide m6A methylome of intestinal porcine epithelial cells (IPEC-J2) induced by deoxynivalenol

Project description:Deoxynivalenol (DON) frequently detected in a wide range of foods and feeds, inducing cytotoxicity to animals and humans. N6-methyladenosine (m6A) is an important epitranscriptomic marker with high abundance in eukaryotic mammals mRNAs. However, the role of the m6A methylomes in DON damage is still poorly understood. Here, we investigated the m6A transcriptome-wide profile in intestinal porcine epithelial cells (IPEC-J2) with and without 1000 ng/mL DON treatment via m6A sequencing and RNA sequencing. In total, 5406 new m6A peaks appeared with the disappearance of 2615 peaks in DON-induced IPEC-J2. The unique m6A-modified genes in DON-induced IPEC-J2 were associated with TNF signaling pathway. We identified 733 differentially expressed mRNA transcripts with hyper-methylated or hypo-methylated m6A peaks between DON-induced IPEC-J2 and normal IPEC-J2. Protein interaction network analysis and qPCR validation suggested that CSF2 probably acts as a promising new target for combating DON damage in IPEC-J2. Our first report of m6A transcriptome-wide map of IPEC-J2 cells presented here provides a starting roadmap for uncovering m6A functions that may affect DON infection.

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Effect of antimicrobial peptide PR-39 on the gene expression of porcine intestinal epithelial cell line (IPEC-J2)

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Effect of antimicrobial peptide PR-39 on the gene expression of porcine intestinal epithelial cell line (IPEC-J2)

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To investigate the mechanisms of CDCA regulates proliferation of Porcine Small Intestinal Epithelial Cells (IPEC-J2)

Project description:To investigate the mechanisms of CDCA regulates proliferation of Porcine Small Intestinal Epithelial Cells (IPEC-J2)

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Transcriptome Profile of Natural Product Deoxyshikonin Induced Gene Expression in IPEC-J2

Project description:To understand the immunomodulatory effects of deoxyshikonin (a natural derivative of well-known Chinese medicine shikonin) on porcine intestinal epithelium cells, the transcriptome analysis was performed to explore the transcriptional profile of porcine IPEC-J2 cells after deoxyshikonin treatment.

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Gene Regulation of Intestinal Porcine Epithelial Cells IPEC‑J2 is Dependent on the Site of Deoxynivalenol Toxicological Action

Project description:Here we analysed different mechanisms of apical and basolateral deoxynivalenol (DON) toxicity reflected in the gene expression. We used the jejunum derived, polarized intestinal porcine epithelial cell line IPEC‑J2 as an in vitro cell culture model. Luminal and blood stream DON intoxication was mimicked by a DON application from the apical or basolateral compartment of ThinCert® membrane inserts for 72 h.

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