Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Characterization of transcriptome in post-replicative mouse male germ cells

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Mus musculus

PROVIDER: GSE214316 | GEO | 2023/12/04

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Characterization of transcriptome in post-replicative mouse male germ cells [RNA-seq]

Project description:To investigate the complexity of RNA present in different stage germ cells from adult mice, we isolated cells via FACS We then performed paired end RNA-Seq to characterize the expression state of each cell type

2023-12-04 | GSE214315 | GEO

Characterization of transcriptome in post-replicative mouse male germ cells [ChIP-Seq]

Project description:To investigate transcriptional changes in developing mouse testicular germ cells, cells were isolated via FACS or centrifugal elutriation Isolated cells were subjected to native ChIP-Seq with an antibody against H3K4me3

2023-12-04 | GSE214313 | GEO

Dual DNA staining enables isolation of multiple sub-types of post-replicative mouse male germ cells.

Project description:During spermatogenesis, mammalian male germ cells undergo multiple developmental processes, including meiosis and post-meiotic differentiation (spermiogenesis). To understand the transitions between different cellular states it is essential to isolate pure populations of cells at different stages of development. Previous approaches enabled the isolation of cells from different stages of meiotic prophase I, but techniques to sub-fractionate unfixed, post-meiotic spermatids have been lacking. Here we report the development of a protocol enabling simultaneous isolation of cells at different stages of meiotic prophase and post-meiotic differentiation from testes of adult mice. This approach builds on existing fluorescence activated cell sorting protocols designed to purify cells in different stages of meiotic prophase I. By utilizing the specific spectral properties that two different DNA dyes (Hoechst 33342 and SYTO 16) exhibit when bound to chromatin of different stage male germ cells, we obtain highly pure populations of cells in relatively large numbers. This FACS protocol will enable immunocytological and molecular characterization studies of fractionated meiotic and haploid germ cells from both wild type and genetically mutant animals.

| S-EPMC9303198 | biostudies-literature

Repeat RNAs associate with replication forks and post-replicative DNA

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-03-26 | GSE139353 | GEO

In Vitro Reconstitution of the Whole Male Germ-Cell Development from Mouse Pluripotent Stem Cells

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-09-30 | GSE168222 | GEO

Induction of the mouse germ cell fate by transcription factors in vitro

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2013-08-04 | E-GEOD-46855 | biostudies-arrayexpress

Transcriptome characterization of social stressed C57B6 mice exhibiting PTSD like features

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2015-04-20 | E-GEOD-68077 | biostudies-arrayexpress

Characterization of transcriptome dynamics in response to contact with host cells

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2014-03-30 | E-GEOD-56093 | biostudies-arrayexpress

RNA-seq and H3K9me2 ChIP-seq of postnatal male germ cells

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-06-08 | GSE148055 | GEO

Default DNA Methylation is Preceded by Broad, Low-Level Transcription in Fetal Male Germ Cells and Is Inversely Patterned by Dynamic H3K4 Methylation

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2013-07-31 | E-GEOD-46954 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data